Novel Allelic Gene Variations in CmCLAVATA3 (CmCLV3) Were Identified in a Genetic Population of Melon (Cucumis melo L.)
Abstract
:1. Introduction
2. Results
2.1. Genetic and Phenotypic Analysis of Melon Carpel Number
2.2. Localization of the Number of Melon Carpels Gene
2.3. Identification of Candidate Genes and Study of Mutation Mechanism
2.4. Cis-Trans Test for Polycarpous Candidate Genes
2.5. Analysis of the Expression Patterns of Candidate Genes and Prediction of Protein Structures
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Phenotypic Analysis
4.2. Constructing a Phylogenetic Tree
4.3. BSA Analysis and Initial Mapping
4.4. Fine-Mapping
4.5. Expression Analysis and Prediction of Protein Structures
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Generations | Plants | Normal-CN (Mean) | Multi-CN (Mean) | Expected Ratio | Actual Ratio | χ2 | p Value |
---|---|---|---|---|---|---|---|
W336(P1) | 14 | 14 (3.05) | 0 | ||||
CR465(P2) | 33 | 0 | 33 (4.49) | ||||
F1 | 17 | 17 (3.33) | 0 | ||||
F2-2022 | 178 | 142 (3.18) | 41 (4.31) | 3:1 | 3.46:1 | 0.667 | 0.412 |
Sample Name | CmCLV3H1 | CmCLV3H2 | Carpel Number (Mean) |
---|---|---|---|
A-F1 × ivf05 F1-1 | C/G | A/A | normal |
A-F1 × ivf05 F1-2 | C/G | A/T | multi |
A-F1 × ivf05 F1-3 | C/G | A/T | multi |
A-F1 × ivf05 F1-4 | C/G | A/T | multi |
A-F1 × ivf05 F1-5 | C/G | A/T | multi |
A-F1 × ivf05 F1-6 | C/G | A/T | multi |
A-F1 × ivf05 F1-7 | C/G | A/T | multi |
B-F1 × ivf05 F1-1 | C/G | A/A | normal |
B-F1 × ivf05 F1-2 | C/G | A/T | multi |
B-F1 × ivf05 F1-3 | C/G | A/A | normal |
B-F1 × ivf05 F1-4 | C/G | A/T | multi |
B-F1 × ivf05 F1-5 | C/G | A/T | multi |
B-F1 × ivf05 F1-6 | C/G | A/T | multi |
B-F1 × ivf05 F1-7 | C/G | A/A | normal |
B-F1 × ivf05 F1-8 | C/G | A/T | multi |
B-F1 × ivf05 F1-9 | C/G | A/A | normal |
B-F1 × ivf05 F1-10 | C/G | A/A | normal |
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Wu, H.; Jia, Y.; Chen, X.; Jiang, N.; Zhang, Z.; Chai, S. Novel Allelic Gene Variations in CmCLAVATA3 (CmCLV3) Were Identified in a Genetic Population of Melon (Cucumis melo L.). Int. J. Mol. Sci. 2024, 25, 6011. https://doi.org/10.3390/ijms25116011
Wu H, Jia Y, Chen X, Jiang N, Zhang Z, Chai S. Novel Allelic Gene Variations in CmCLAVATA3 (CmCLV3) Were Identified in a Genetic Population of Melon (Cucumis melo L.). International Journal of Molecular Sciences. 2024; 25(11):6011. https://doi.org/10.3390/ijms25116011
Chicago/Turabian StyleWu, Hangyu, Yue Jia, Xinxiu Chen, Naiyu Jiang, Zhonghua Zhang, and Sen Chai. 2024. "Novel Allelic Gene Variations in CmCLAVATA3 (CmCLV3) Were Identified in a Genetic Population of Melon (Cucumis melo L.)" International Journal of Molecular Sciences 25, no. 11: 6011. https://doi.org/10.3390/ijms25116011