Absent in Melanoma 2 Mediates Inflammasome Signaling Activation against Clostridium perfringens Infection
Abstract
:1. Introduction
2. Results
2.1. AIM2 Contributes to Host Protection against Pathogenic C. perfringens Gas Gangrene
2.2. Aim2 Deficiency Leads to Impaired Inflammasome Signaling Activation
2.3. AIM2 Promotes Inflammasome Signaling Activation in Macrophages
2.4. AIM2-Mediated Inflammasome Signaling Facilitates Bacterial Killing and Clearance
2.5. Pathogenic C. perfringens-Derived Genomic DNA Mediates Activation of AIM2 Inflammasome Signaling
3. Discussion
4. Materials and Methods
4.1. Mice and Cell
4.2. Chemical
4.3. In Vivo Infection
4.4. Bacterial Burden and Cytokine Measurements
4.5. Tissue Histology and Immunofluorescence Staining
4.6. Inflammasome Activation Assay
4.7. Bacterial Killing Analysis
4.8. Administration of Exogenous rIL-18
4.9. Immunoblotting
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ma, Z.; Lou, Y.; Wang, N.; Zhao, Y.; Zhang, S.; Zhang, M.; Li, J.; Xu, Q.; He, A.; Yu, S. Absent in Melanoma 2 Mediates Inflammasome Signaling Activation against Clostridium perfringens Infection. Int. J. Mol. Sci. 2024, 25, 6571. https://doi.org/10.3390/ijms25126571
Ma Z, Lou Y, Wang N, Zhao Y, Zhang S, Zhang M, Li J, Xu Q, He A, Yu S. Absent in Melanoma 2 Mediates Inflammasome Signaling Activation against Clostridium perfringens Infection. International Journal of Molecular Sciences. 2024; 25(12):6571. https://doi.org/10.3390/ijms25126571
Chicago/Turabian StyleMa, Zhaoguo, Yanan Lou, Na Wang, Yi Zhao, Shuxin Zhang, Mingyue Zhang, Jiaqi Li, Qian Xu, Aobo He, and Shuixing Yu. 2024. "Absent in Melanoma 2 Mediates Inflammasome Signaling Activation against Clostridium perfringens Infection" International Journal of Molecular Sciences 25, no. 12: 6571. https://doi.org/10.3390/ijms25126571