Autonomous Oscillatory Mitochondrial Respiratory Activity: Results of a Systematic Analysis Show Heterogeneity in Different In Vitro-Synchronized Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Mitochondrial Respiration Undergoes Autonomous Oscillation in In Vitro Serum-Shocked Synchronized Cells
2.2. Rhythmic Mitochondrial Respiration Depends on Bmal1 Expression
2.3. The Autonomous Oscillatory Profile of Mitochondrial Respiration Differs among Different Cell Types
3. Discussion
4. Materials and Methods
4.1. Cells and Culture Conditions
4.2. In Vitro Circadian Rhythms Synchronization
4.3. Respirometric Measurements
4.4. BMAL1 Silencing in HepG2 Cells
4.5. Western Blotting Analysis
4.6. Cosinor Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Organ | Type | Mutated Genes |
---|---|---|---|
HAEC | Aorta | Endothelial | N.A. |
NHDF | Skin | Fibroblast | N.A. |
Cal27 | Tongue | Squamous cell carcinoma | TP53, TERT, CDKN2A, KMT2A, LRBP1 |
Hela | Cervix | Adenocarcinoma | HPV E6 E7, MED1, ERBB3 CASP8, HLA-A, TGFBR2 |
HepG2 | Liver | Epithelial carcinoma | TP53, TERT promoter, CTNNB1 |
SCC9 | Tongue | Squamous cell carcinoma | TP53, TERT, CDKN2A, KMT2A, LRBP1, HIF1A |
SW480 | Colon | Adenocarcinoma | APC, TP53, ERBB2, KRAS |
SW620 | Colon | Adenocarcinoma (met) | APC, TP53, KRAS |
U2OS | Bone | Epithelial osteosarcoma | RB, TP53, RECQLC2, RECQLC3, RECQLC4 |
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Cela, O.; Scrima, R.; Pacelli, C.; Rosiello, M.; Piccoli, C.; Capitanio, N. Autonomous Oscillatory Mitochondrial Respiratory Activity: Results of a Systematic Analysis Show Heterogeneity in Different In Vitro-Synchronized Cancer Cells. Int. J. Mol. Sci. 2024, 25, 7797. https://doi.org/10.3390/ijms25147797
Cela O, Scrima R, Pacelli C, Rosiello M, Piccoli C, Capitanio N. Autonomous Oscillatory Mitochondrial Respiratory Activity: Results of a Systematic Analysis Show Heterogeneity in Different In Vitro-Synchronized Cancer Cells. International Journal of Molecular Sciences. 2024; 25(14):7797. https://doi.org/10.3390/ijms25147797
Chicago/Turabian StyleCela, Olga, Rosella Scrima, Consiglia Pacelli, Michela Rosiello, Claudia Piccoli, and Nazzareno Capitanio. 2024. "Autonomous Oscillatory Mitochondrial Respiratory Activity: Results of a Systematic Analysis Show Heterogeneity in Different In Vitro-Synchronized Cancer Cells" International Journal of Molecular Sciences 25, no. 14: 7797. https://doi.org/10.3390/ijms25147797