Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates
Abstract
:1. Introduction
2. Results
2.1. Focal Adhesion Maturation Is Enhanced in Cultured Human CSFs on FCF
2.2. FCF Induces Changes in Cell Matrix Interaction-Related Protein Expression and Early Mechanotransduction in Cultured Human CSFs
2.3. pFAK Inhibition Overrules Early Mechanotransduction in Human CSFs Cultured on FCF
3. Discussion
4. Materials and Methods
4.1. Human Tissue Sourcing
4.2. Isolation and Culture of Human Corneal Stromal Fibroblasts
4.3. Flow Cytometry
4.4. Human Amniotic Membrane Preparation
4.5. Fibrillar Collagen Film Fabrication
4.6. Scanning Electron Microscopy
4.7. Immunostaining
4.8. Quantitative Immunofluorescence Analysis
4.9. Quantitative Real-Time Polymerase Chain Reaction
4.10. Cell Motility Analysis
4.11. Proteomic Profiling
4.12. Y397-FAK Autophosphorylation Inhibition
4.13. Western Blot Analysis
4.14. Cell Viability Assay
4.15. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhurenkov, K.E.; Lobov, A.A.; Bildyug, N.B.; Alexander-Sinclair, E.I.; Darvish, D.M.; Lomert, E.V.; Kriger, D.V.; Zainullina, B.R.; Chabina, A.S.; Khorolskaya, J.I.; et al. Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates. Int. J. Mol. Sci. 2024, 25, 8601. https://doi.org/10.3390/ijms25168601
Zhurenkov KE, Lobov AA, Bildyug NB, Alexander-Sinclair EI, Darvish DM, Lomert EV, Kriger DV, Zainullina BR, Chabina AS, Khorolskaya JI, et al. Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates. International Journal of Molecular Sciences. 2024; 25(16):8601. https://doi.org/10.3390/ijms25168601
Chicago/Turabian StyleZhurenkov, Kirill E., Arseniy A. Lobov, Natalya B. Bildyug, Elga I. Alexander-Sinclair, Diana M. Darvish, Ekaterina V. Lomert, Daria V. Kriger, Bozhana R. Zainullina, Alina S. Chabina, Julia I. Khorolskaya, and et al. 2024. "Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates" International Journal of Molecular Sciences 25, no. 16: 8601. https://doi.org/10.3390/ijms25168601