Emergence and Comparative Genome Analysis of Salmonella Ohio Strains from Brown Rats, Poultry, and Swine in Hungary
Abstract
:1. Introduction
2. Results
2.1. Prevalence and Distribution of S. Ohio Strains in Brown Rats and Food-Producing Animals
2.2. Phenotypic Features of S. Ohio Strains from Hungary
2.3. Comparison of the Genomic Determinants of Virulence and Antimicrobial Resistance
2.4. Phylogenetic Relation of S. Ohio Strains with Different Geographical Distributions
3. Discussion
4. Materials and Methods
4.1. Samples Collected from Brown Rats
4.2. Sample Processing, Detection, and Identification of Salmonella Strains
4.3. Antibiotic Susceptibility Testing of Salmonella Ohio Strains and Selection for Genome Analysis
4.4. Whole-Genome Sequencing and Prediction of the Antibiotic Resistance and Virulence Genotypes
4.5. Phylogenetic Analysis: MLST and Core Genome MLST
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Animal/Source | Total Tested | Prevalence (n) | Strains |
---|---|---|---|
Brown rat | n = 92 | 4.3% (4) | 502/S1; 503/S1; 506/S1; 512/S1 * |
Broiler chicken (2022) a | n = 17,134 | 6‰ (1) | SM-230/22 * |
Fattening turkey (2022) a | n = 3593 | 0.056% (2) | SM-804/22 *; SM-805/22 |
Goose (2021) b | n = 3593 | 0.056% (2) | 194/21 *; 304/21; |
Goose (2022) b | n = 130 | 1.5% (2) | 420/22; 421/22 |
Swine (2022) b | n = 48 | 2.0% (1) | 453/22 * |
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Szmolka, A.; Lancz, Z.S.; Rapcsák, F.; Egyed, L. Emergence and Comparative Genome Analysis of Salmonella Ohio Strains from Brown Rats, Poultry, and Swine in Hungary. Int. J. Mol. Sci. 2024, 25, 8820. https://doi.org/10.3390/ijms25168820
Szmolka A, Lancz ZS, Rapcsák F, Egyed L. Emergence and Comparative Genome Analysis of Salmonella Ohio Strains from Brown Rats, Poultry, and Swine in Hungary. International Journal of Molecular Sciences. 2024; 25(16):8820. https://doi.org/10.3390/ijms25168820
Chicago/Turabian StyleSzmolka, Ama, Zsuzsanna Sréterné Lancz, Fanni Rapcsák, and László Egyed. 2024. "Emergence and Comparative Genome Analysis of Salmonella Ohio Strains from Brown Rats, Poultry, and Swine in Hungary" International Journal of Molecular Sciences 25, no. 16: 8820. https://doi.org/10.3390/ijms25168820