Dynamics of Actin Filaments Play an Important Role in Root Hair Growth under Low Potassium Stress in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Low K+ Stress Prolongs Root Hair Growth Time and Improves Root Hair Growth Rate
2.2. Dynamics of Actin Filaments Are Affected in Root Hair Growth under Low K+ Stress
2.3. The Expression of VLN1 and VLN4 in Root Hairs Is Affected under Low K+ Stress
2.4. VLN1 and VLN4 Regulate Root Hair Growth under Low K+ Stress
2.5. VLN1 and VLN4 Regulate Dynamics of Actin Filaments in Root Hair Growth under Low K+ Stress
2.6. VLN1 and VLN4 Affect the Seedlings Growth under Low K+ Stress
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Plant Growth Conditions and the Measurement of Growth Status
5.2. Plasmid Construction and Plant Transformation
5.3. Observation and Analysis of Root Hair Growth
5.4. Gene Expression and GUS Activity Analysis
5.5. Western Blot Assays
5.6. Quantitative Analysis of Actin Arrays
5.7. Time-Lapse Imaging of Signal Actin Filament Dynamics
5.8. Potassium Sensitivity Analysis
5.9. Potassium Content Measurement
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Li, M.; Liu, S.; Wang, J.; Cheng, X.; Diao, C.; Yan, D.; Gao, Y.; Wang, C. Dynamics of Actin Filaments Play an Important Role in Root Hair Growth under Low Potassium Stress in Arabidopsis thaliana. Int. J. Mol. Sci. 2024, 25, 8950. https://doi.org/10.3390/ijms25168950
Li M, Liu S, Wang J, Cheng X, Diao C, Yan D, Gao Y, Wang C. Dynamics of Actin Filaments Play an Important Role in Root Hair Growth under Low Potassium Stress in Arabidopsis thaliana. International Journal of Molecular Sciences. 2024; 25(16):8950. https://doi.org/10.3390/ijms25168950
Chicago/Turabian StyleLi, Mingyang, Shihang Liu, Jinshu Wang, Xin Cheng, Chengxuan Diao, Dabo Yan, Yue Gao, and Che Wang. 2024. "Dynamics of Actin Filaments Play an Important Role in Root Hair Growth under Low Potassium Stress in Arabidopsis thaliana" International Journal of Molecular Sciences 25, no. 16: 8950. https://doi.org/10.3390/ijms25168950