Effects of Fasting on THP1 Macrophage Metabolism and Inflammatory Profile
Abstract
:1. Introduction
2. Results
2.1. SD Modulates Macrophage Phenotype and Its Response to LPS Stimulation
2.2. SD Affects Macrophage Metabolic Activity
2.3. MTS Reduction and Macrophage Profile Correlate to Metabolic Changes
3. Discussion
4. Materials and Methods
4.1. Cell Line
4.2. Cell Treatments
4.3. Cell Counting
4.4. MTS Assay
4.5. RT-PCR and qPCR
4.6. Immunofluorescence
4.7. NAD+ and NADH Quantitation
4.8. Phospho-AMPK Alpha 1 and Total AMPK Alpha 1
4.9. Reactive Oxygen Species
4.10. Mitochondrial Membrane Potential
4.11. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Rius-Bonet, J.; Macip, S.; Massip-Salcedo, M.; Closa, D. Effects of Fasting on THP1 Macrophage Metabolism and Inflammatory Profile. Int. J. Mol. Sci. 2024, 25, 9029. https://doi.org/10.3390/ijms25169029
Rius-Bonet J, Macip S, Massip-Salcedo M, Closa D. Effects of Fasting on THP1 Macrophage Metabolism and Inflammatory Profile. International Journal of Molecular Sciences. 2024; 25(16):9029. https://doi.org/10.3390/ijms25169029
Chicago/Turabian StyleRius-Bonet, Julia, Salvador Macip, Marta Massip-Salcedo, and Daniel Closa. 2024. "Effects of Fasting on THP1 Macrophage Metabolism and Inflammatory Profile" International Journal of Molecular Sciences 25, no. 16: 9029. https://doi.org/10.3390/ijms25169029