Multi-Omics Integrative Analysis to Reveal the Impacts of Shewanella algae on the Development and Lifespan of Marine Nematode Litoditis marina
Abstract
:1. Introduction
2. Results
2.1. S. algae Delayed Development and Shortened Lifespan of L. marina
2.2. Transcriptomic Analysis of L. marina Feeding with S. algae versus E. coli OP50
2.3. Transcriptomic Analysis of S. algae versus E. coli OP50
2.4. Metabolic Profiling of S. algae- and E. coli OP50-Conditioned Media
2.5. Joint Pathway Analysis of DAMs and DEGs in S. algae versus E. coli OP50
3. Discussion
3.1. Higher Expression of Genes in Iron Ion Binding and Oxidoreductase Activity Pathways Might Promote Aging of L. marina When Grown on S. algae
3.2. Decreased Expression of Extracellular Structural Components-Related Genes Might Delay the Development of L. marina fed with S. algae
3.3. Downregulated Bacterial Amino Acid Metabolism Genes in S. algae Might Delay Development and Shorten the Lifespan of L. marina
3.4. Higher Expression of Virulence-Related Genes in S. algae Might Contribute to the Delayed Development and Shortened Lifespan of L. marina
3.5. Impact of Toxic Metabolites and Nucleotide on L. marina Development and Lifespan
3.6. “Two-Component System” and “One-Carbon Pool by Folate” Pathways Might Modulate L. marina Physiology
4. Materials and Methods
4.1. Worm and Culture
4.2. Bacteria
4.3. Development and Lifespan Assays
4.4. Sample Collection
4.5. RNA Library Preparation, RNA Sequencing and Transcriptional Analysis of L. marina
4.6. RNA Library Preparation, RNA Sequencing, and Transcriptional Analysis of Bacteria
4.7. Metabolomics
4.8. Bacterial Transcriptome and Metabolism Conjoint Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xue, Y.; Xue, B.; Zhang, L. Multi-Omics Integrative Analysis to Reveal the Impacts of Shewanella algae on the Development and Lifespan of Marine Nematode Litoditis marina. Int. J. Mol. Sci. 2024, 25, 9111. https://doi.org/10.3390/ijms25169111
Xue Y, Xue B, Zhang L. Multi-Omics Integrative Analysis to Reveal the Impacts of Shewanella algae on the Development and Lifespan of Marine Nematode Litoditis marina. International Journal of Molecular Sciences. 2024; 25(16):9111. https://doi.org/10.3390/ijms25169111
Chicago/Turabian StyleXue, Yiming, Beining Xue, and Liusuo Zhang. 2024. "Multi-Omics Integrative Analysis to Reveal the Impacts of Shewanella algae on the Development and Lifespan of Marine Nematode Litoditis marina" International Journal of Molecular Sciences 25, no. 16: 9111. https://doi.org/10.3390/ijms25169111