Unraveling the Molecular Mechanisms by Which the miR171b-SCL6 Module Regulates Maturation in Lilium
Abstract
:1. Introduction
2. Results
2.1. Bioinformatics Analysis of Lbr-miR171b and LbrSCL6
2.2. Validation of qRT-PCR for Lbr-miR171b and LbrSCL6
2.3. Identification of the Targeting Relationship between Lbr-miR171b and LbrSCL6
2.4. Subcellular Localization and Transcriptional Activation of LbrSCL6 and LbrWOX4
2.5. In Situ Hybridization of LbrSCL6
2.6. Transgenic Functional Validation of Lbr-miR171b and LbrSCL6
2.7. Bimolecular Fluorescence Complementation of LbrSCL6 and LbrWOX4
2.8. Yeast Two-Hybridization of LbrSCL6 and LbrWOX4
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Bioinformatics Analysis
4.3. qRT-PCR Analysis
4.4. Gene Cloning
4.5. Validation of the Targeting Relationship between Lbr-miR171b and LbrSCL6
4.6. Subcellular Localization and Transcriptional Activation
4.7. In Situ Hybridization
4.8. Transgenic Functional Verification
4.9. Bimolecular Fluorescence Complementation and Yeast Two-Hybrid Assays
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, Q.; Song, M.; Wang, Y.; Lu, P.; Ge, W.; Zhang, K. Unraveling the Molecular Mechanisms by Which the miR171b-SCL6 Module Regulates Maturation in Lilium. Int. J. Mol. Sci. 2024, 25, 9156. https://doi.org/10.3390/ijms25179156
Li Q, Song M, Wang Y, Lu P, Ge W, Zhang K. Unraveling the Molecular Mechanisms by Which the miR171b-SCL6 Module Regulates Maturation in Lilium. International Journal of Molecular Sciences. 2024; 25(17):9156. https://doi.org/10.3390/ijms25179156
Chicago/Turabian StyleLi, Qing, Meiqi Song, Yachen Wang, Ping Lu, Wei Ge, and Kezhong Zhang. 2024. "Unraveling the Molecular Mechanisms by Which the miR171b-SCL6 Module Regulates Maturation in Lilium" International Journal of Molecular Sciences 25, no. 17: 9156. https://doi.org/10.3390/ijms25179156