Apoptosis–Cell Cycle–Autophagy Molecular Mechanisms Network in Heterogeneous Aggressive Phenotype Prostate Hyperplasia Primary Cell Cultures Have a Prognostic Role
, Manuela Enciu 2,3,*, Mihai Cătălin Roșu 1,†, Felix Voinea 3,4, Anca Florentina Mitroi 1,2, Mariana Deacu 2,3, Gabriela Isabela Băltățescu 1,2, Antonela-Anca Nicolau 1,2, Anca Chisoi 1, Mariana Aşchie 2,3 and Anita Cristina Ionescu (Mitu) 3,5Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThis study was done and conducted solely by flow cytometry, and it is difficult and not sufficient to explain multiple biological characteristics. Authors are advised to perform additional experiments to support the results. For instance, L124-126, “cell cycle arrest in G2/M phase being characterized by aneuploidy status”. The aneuploidy status requires further evidence to prove.
The results of Fig.2 lack of descriptive explanation.
How would authors explain the BPH cell culture S5 showed a significantly high percentage both at the S phase and G2/M phase?
Comments on the Quality of English LanguageSome typing mistakes and words are incorrectly chosen in the manuscript, such as intern control, reported to control.
Author Response
Dear Reviewer 1,
Please see the attachment.
Best regards,
PhD Biologist, Researcher III, Matei Elena
CEDMOG, Ovidius University from Constanta, Romania
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsIn this manuscript, the authors investigated cell death, DNA content, and autophagy molecular mechanisms network as predictor biomarkers to highlight prostate pathogenesis and its prognostic role. This study is based on flow cytometric analysis.
The authors observed that higher late apoptosis expression, high proliferative S phase of cell cycle category, and aneuploidy status represent unfavorable prognostic biomarkers and dependent predictor factors in heterogeneous aggressive phenotype BHP cell cultures.
Overall, this study is potentially of interest. However, as the results are only based on flow cytometric analysis, different methodology approaches (including WB and IHC) are required to confirm the results and support the conclusions.
Specific comments:
Figure 2 shows nodular hyperplasia, atrophya, and chronic inflammation in primary prostate cell cultures developed from tissue pieces recovered from patients with hyperplasia (BPH). The images are not convincing. Immunohistochemistry (IHC) staining is required using specific markers to study inflammatory cells in the TME.
Comments on the Quality of English LanguageMinor editing of English language is required.
Author Response
Dear Reviewer 2,
Please see the attachment.
Best regards,
PhD Biologist, Researcher III, Matei Elena
CEDMOG, Ovidius University from Constanta, Romania
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Comments and Suggestions for AuthorsThe manuscript has been revised. No further comments.
Comments on the Quality of English LanguageSome typing mistakes in text.
Author Response
Dear Reviewer 1,
Please see the attachment.
Best regards,
PhD Biologist, Researcher III, Matei Elena
CEDMOG, Ovidius University from Constanta, Romania
Author Response File: Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsAlthough the authors partially addressed the raised questions, the new version is sufficiently improved.
Author Response
Dear Reviewer 2,
Please see the attachment.
Best regards,
PhD Biologist, Researcher III, Matei Elena
CEDMOG, Ovidius University from Constanta, Romania
Author Response File: Author Response.pdf
