Kynurenic Acid Modulates the Expression of Genes and the Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep
Abstract
:1. Introduction
2. Results
2.1. SOD2 Expression Levels and Activity
2.2. CAT Expression Levels and Activity
2.3. GPx1 Expression Level and Activity
3. Discussion
4. Materials and Methods
4.1. Animal Maintenance
4.2. Third Ventricle Cannulation
4.3. Experimental Design and Tissue Collection
4.4. Relative mRNA Abundance
4.5. Determination of Antioxidant Enzyme Activity
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primers (5′–3′) | Genbank Acc. No. | Amplicon Size |
---|---|---|---|
SOD2 | F: GCAAGGAACAACAGGTCTTATCC R: ACTTGGTGTAAGGCTGACGG | NM_001280703.1 | 181 |
CAT | F: GAGCCCACCTGCAAAGTTCT R: CTCCTACTGGATTACCGGCG | XM_004016396.6 | 148 |
GPx1 | F: TGTCGTACTCGGCTTCCC R: AGCGGATGCGCCTTCTCG | XM_004018462.1 | 163 |
GAPDH | F: GGGTCATCATCTCTGCACCT R: GGTCATAAGTCCCTCCACGA | NM_001190390.1 | 131 |
PPIC | F: TGGAAAAGTCGTGCCCAAGA R: TGCTTATACCACCAGTGCCA | XM_004008676.1 | 158 |
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Misztal, T.; Roszkowicz-Ostrowska, K.; Kowalczyk, P.; Młotkowska, P.; Marciniak, E. Kynurenic Acid Modulates the Expression of Genes and the Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep. Int. J. Mol. Sci. 2024, 25, 9428. https://doi.org/10.3390/ijms25179428
Misztal T, Roszkowicz-Ostrowska K, Kowalczyk P, Młotkowska P, Marciniak E. Kynurenic Acid Modulates the Expression of Genes and the Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep. International Journal of Molecular Sciences. 2024; 25(17):9428. https://doi.org/10.3390/ijms25179428
Chicago/Turabian StyleMisztal, Tomasz, Katarzyna Roszkowicz-Ostrowska, Paweł Kowalczyk, Patrycja Młotkowska, and Elżbieta Marciniak. 2024. "Kynurenic Acid Modulates the Expression of Genes and the Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep" International Journal of Molecular Sciences 25, no. 17: 9428. https://doi.org/10.3390/ijms25179428