The Unexplored Role of Mitochondria-Related Oxidative Stress in Diverticular Disease
Abstract
:1. Introduction
2. Results
2.1. Human Tissue Collection
2.2. Ultrastructural Analyses of Mitochondria Smooth Muscle Tissues
2.3. Mitochondrial Respiratory Chain
2.3.1. Mitochondrial Respiratory Chain-Complex II (SDHA)
2.3.2. Mitochondrial Respiratory Chain—Complex IV (COX)
2.4. Mitochondrial Alterations and Antioxidant Status Evaluation in Isolated Smooth Muscle Cells
2.4.1. Mitochondrial Mass
2.4.2. Mitochondrial Membrane Potential (Δψm)
2.4.3. Glutathione Intracellular Content
2.5. Functional Activity of Isolated Smooth Muscle Cells
2.6. Functional Effects of N-Acetylcysteine
3. Discussion
4. Materials and Methods
4.1. Patients and Colonic Specimens
4.2. Cellular Ex Vivo Mode
4.3. Transmission Electron Microscopy (TEM)
4.4. Western Blot
4.5. Flow Cytometry Analysis
4.6. Functional Study
4.7. Antioxidant In Vitro Model
4.8. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Controls | Diverticulosis | Complicated DD | |
---|---|---|---|
Gender | 7 women/6 men | 8 women/9 men | 8 women/7 men |
Age (years) median (95% CI) | 68 (53–92) | 76 (46–89) | 67 (42–85) |
BMI (kg/m2) median (95% CI) | 23 (21–30) | 24.0 (21–29) | 26.0 (20–34) |
Contraction | Relaxation | |
---|---|---|
LONGITUDINAL layer | ||
DIV | 45.7% ± 7.1 | 56.9% * ± 5.3 |
cDD | 61.7% * ± 9.6 | 56.9% * ± 5.3 |
CIRCULAR layer | ||
DIV | 46.7% ± 5.8 | 21.9% ± 10.2 |
cDD | 56.4% ± 14.2 | 54.6% ± 13.6 |
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Cappelletti, M.; Pallotta, L.; Vona, R.; Tinari, A.; Pisano, A.; Casella, G.; Crocetti, D.; Carlomagno, D.; Tattoli, I.; Giordano, C.; et al. The Unexplored Role of Mitochondria-Related Oxidative Stress in Diverticular Disease. Int. J. Mol. Sci. 2024, 25, 9680. https://doi.org/10.3390/ijms25179680
Cappelletti M, Pallotta L, Vona R, Tinari A, Pisano A, Casella G, Crocetti D, Carlomagno D, Tattoli I, Giordano C, et al. The Unexplored Role of Mitochondria-Related Oxidative Stress in Diverticular Disease. International Journal of Molecular Sciences. 2024; 25(17):9680. https://doi.org/10.3390/ijms25179680
Chicago/Turabian StyleCappelletti, Martina, Lucia Pallotta, Rosa Vona, Antonella Tinari, Annalinda Pisano, Giovanni Casella, Daniele Crocetti, Dominga Carlomagno, Ivan Tattoli, Carla Giordano, and et al. 2024. "The Unexplored Role of Mitochondria-Related Oxidative Stress in Diverticular Disease" International Journal of Molecular Sciences 25, no. 17: 9680. https://doi.org/10.3390/ijms25179680