PRC1 Protein Subcomplexes Architecture: Focus on the Interplay between Distinct PCGF Subunits in Protein Interaction Networks
Abstract
:1. Introduction
2. Results
2.1. A Physical Interaction Screen for PRC1 Components Purified under Endogenous Conditions
2.2. Affinity Purification (AP)-MS Screening Reveals Common and Distinct Interactomes among PRC1–PCGF Complexes
2.3. Relative Abundance Estimation and Molecular Mass of the Isolated PRC1 Complexes
2.4. Functional Enrichment of PCGF Interactome Map to Multiple Pathways
2.5. The Role of PCGF Subunits in NT2 Cells and Mutual Regulation of PCGF Variants
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Isolation of Nuclei
4.3. Immunoprecipitation
4.4. Mass Spectrometry Analysis
4.5. Data Processing
4.6. Data and Statistical Analysis
4.7. Immunoblotting
4.8. Gel Filtration Column Chromatography
4.9. Real-Time Quantitative PCR
4.10. Cell Viability Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Munawar, N.; Wynne, K.; Oliviero, G. PRC1 Protein Subcomplexes Architecture: Focus on the Interplay between Distinct PCGF Subunits in Protein Interaction Networks. Int. J. Mol. Sci. 2024, 25, 9809. https://doi.org/10.3390/ijms25189809
Munawar N, Wynne K, Oliviero G. PRC1 Protein Subcomplexes Architecture: Focus on the Interplay between Distinct PCGF Subunits in Protein Interaction Networks. International Journal of Molecular Sciences. 2024; 25(18):9809. https://doi.org/10.3390/ijms25189809
Chicago/Turabian StyleMunawar, Nayla, Kieran Wynne, and Giorgio Oliviero. 2024. "PRC1 Protein Subcomplexes Architecture: Focus on the Interplay between Distinct PCGF Subunits in Protein Interaction Networks" International Journal of Molecular Sciences 25, no. 18: 9809. https://doi.org/10.3390/ijms25189809