Identification and Molecular Characterization of the CAMTA Gene Family in Solanaceae with a Focus on the Expression Analysis of Eggplant Genes under Cold Stress
Abstract
:1. Introduction
2. Results
2.1. Identification and Evolution Analysis of CAMTA Family Members
2.2. Gene Structure and Conserved Motif Analysis of CAMTA Family Members
2.3. Analysis of Cis-Regulatory Element of CAMTA Family Promoter
2.4. Expression Profiling of SmCAMTAs Genes in Different Tissues and under Cold Stress
2.5. Protein–Protein Interaction Analysis of SmCAMTAs
2.6. SmCAMTA2 and SmCAMTA6 Regulated the Transcription of Cold-Related CBF Genes under Cold Stress
3. Discussion
4. Methods and Materials
4.1. Plant Materials, Growth Conditions, and Stress Treatments
4.2. Identification of CAMTA Genes
4.3. Phylogenetic Analysis and Classification
4.4. Motif Composition and Gene Structure Analysis
4.5. Analysis of Cis-Acting Elements in the Promoter Region
4.6. Gene Expression Analysis
4.7. RNA Isolation and qRT-PCR Analysis
4.8. Protein–Protein Analysis of SmCAMTAs
4.9. SmCAMTAs Participate in Transcriptional Regulation of SmCBFs
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Gene ID | Name | Subgroup | ORF (bp) | AA | pI | MW (kDa) | Chr |
---|---|---|---|---|---|---|---|---|
Solanum melongena | SMEL4.1_01g027530.1.01 | SmCAMTA1 | Subgroup I | 2769 | 923 | 7.15 | 104.200 | 1 |
SMEL4.1_11g009470.1.01 | SmCAMTA2 | Subgroup III | 3309 | 1103 | 5.43 | 123.588 | 11 | |
SMEL4.1_03g010310.1.01 | SmCAMTA3 | Subgroup II | 2889 | 963 | 5.83 | 107.849 | 3 | |
SMEL4.1_05g010190.1.01 | SmCAMTA4 | Subgroup I | 2865 | 955 | 6.48 | 106.575 | 5 | |
SMEL4.1_05g021930.1.01 | SmCAMTA5 | Subgroup I | 2703 | 901 | 6.78 | 102.294 | 5 | |
SMEL4.1_01g009270.1.01 | SmCAMTA6 | Subgroup III | 3147 | 1049 | 5.65 | 118.563 | 1 | |
Solanum lycopersicum | Solyc01g057270.2.1 | SlCAMTA1 | Subgroup I | 2871 | 957 | 8.92 | 107.785 | 1 |
Solyc01g105230.2.1 | SlCAMTA2 | Subgroup I | 3120 | 1040 | 5.47 | 117.417 | 1 | |
Solyc04g056270.2.1 | SlCAMTA3 | Subgroup III | 3063 | 1021 | 5.76 | 114.146 | 4 | |
Solyc05g015650.2.1 | SlCAMTA4 | Subgroup II | 2805 | 935 | 5.89 | 105.304 | 5 | |
Solyc12g035520.1.1 | SlCAMTA5 | Subgroup II | 2919 | 973 | 6.25 | 108.982 | 12 | |
Solyc12g099340.1.1 | SlCAMTA6 | Subgroup III | 2945 | 915 | 6.74 | 103.939 | 12 | |
Solanum pennellii | Sopen01g021880.1 | SpCAMTA1 | Subgroup I | 2835 | 945 | 9.02 | 106.506 | 1 |
Sopen01g026290.1 | SpCAMTA2 | Subgroup III | 2760 | 920 | 8.07 | 104.079 | 1 | |
Sopen01g047740.1 | SpCAMTA3 | Subgroup I | 3147 | 1049 | 5.45 | 118.478 | 1 | |
Sopen04g025150.1 | SpCAMTA4 | Subgroup I | 3294 | 1098 | 5.51 | 122.826 | 4 | |
Sopen05g011110.1 | SpCAMTA5 | Subgroup II | 2892 | 964 | 5.72 | 108.263 | 5 | |
Sopen12g015500.1 | SpCAMTA6 | Subgroup II | 2919 | 973 | 6.25 | 108.922 | 12 | |
Sopen12g034010.1 | SpCAMTA7 | Subgroup III | 2730 | 910 | 6.66 | 103.230 | 2 | |
Capsicum annuum | CA08g15740 | CaCAMTA1 | Subgroup I | 3054 | 1018 | 5.81 | 114.798 | 8 |
CA11g07570 | CaCAMTA2 | Subgroup II | 3069 | 1023 | 6.19 | 115.200 | 11 | |
CA11g10930 | CaCAMTA3 | Subgroup III | 2355 | 785 | 6.01 | 88.879 | 11 | |
Lycium barbarum | XP_060191074.1 | LbCAMTA1 | Subgroup II | 2838 | 946 | 6.24 | 105.811 | 11 |
XP_060191073.1 | LbCAMTA2 | Subgroup II | 2826 | 962 | 6.04 | 107.508 | 11 | |
XP_060191072.1 | LbCAMTA3 | Subgroup II | 2898 | 966 | 6.13 | 107.783 | 11 | |
XP_060191071.1 | LbCAMTA4 | Subgroup II | 3003 | 1001 | 6.09 | 111.977 | 11 | |
XP_060191070.1 | LbCAMTA5 | Subgroup II | 3024 | 1008 | 6.04 | 112.836 | 11 | |
XP_060191069.1 | LbCAMTA6 | Subgroup II | 3033 | 1011 | 6.09 | 113.150 | 11 |
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Cai, P.; Lan, Y.; Gong, F.; Li, C.; Xia, F.; Li, Y.; Fang, C. Identification and Molecular Characterization of the CAMTA Gene Family in Solanaceae with a Focus on the Expression Analysis of Eggplant Genes under Cold Stress. Int. J. Mol. Sci. 2024, 25, 2064. https://doi.org/10.3390/ijms25042064
Cai P, Lan Y, Gong F, Li C, Xia F, Li Y, Fang C. Identification and Molecular Characterization of the CAMTA Gene Family in Solanaceae with a Focus on the Expression Analysis of Eggplant Genes under Cold Stress. International Journal of Molecular Sciences. 2024; 25(4):2064. https://doi.org/10.3390/ijms25042064
Chicago/Turabian StyleCai, Peng, Yanhong Lan, Fangyi Gong, Chun Li, Feng Xia, Yifan Li, and Chao Fang. 2024. "Identification and Molecular Characterization of the CAMTA Gene Family in Solanaceae with a Focus on the Expression Analysis of Eggplant Genes under Cold Stress" International Journal of Molecular Sciences 25, no. 4: 2064. https://doi.org/10.3390/ijms25042064