Stable Dual miR-143 and miR-506 Upregulation Inhibits Proliferation and Cell Cycle Progression
Abstract
:1. Introduction
2. Results
2.1. Stable Transduction of Cells
2.2. miR-143 Deregulation
2.2.1. Lentiviral Transduction Induced miR-143 Deregulation
2.2.2. miR-143 Upregulation Decreased S Phase Population, but All miR-143 Deregulations Increased G2 Phase Population, without Affecting Cell Proliferation
2.2.3. The Two miR-143 Deregulations Altered Cell Morphology and Motility in a Similar Manner
2.3. miR-506 Deregulation
2.3.1. Lentiviral Transduction Induced miR-506 Deregulation
2.3.2. miR-506 Downregulation, but Not Upregulation, Affected the Cell Cycle, but Both Slowed Down Proliferation
2.3.3. The Two miR-506 Deregulations Altered Cell Morphology in a Similar Manner, but Not Motility
2.4. miR-506 and miR-143 Deregulation
2.4.1. Dual Lentiviral Transduction Induced Both miR-506 and miR-143 Deregulation
2.4.2. miR-143/506 Upregulation Increased G2 Cell Population and Slowed Down Proliferation
2.4.3. miR-143/506 Upregulation Altered Cell Morphology, but miR-143/506 Downregulation Increased Cell Motility
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Stable Cell Line Generation
4.4. Cell Sorting
4.5. RNA Extraction and qPCR Analysis
4.6. Cell Cycle Analysis
4.7. Wound Healing Assay
4.8. Mycoplasma Detection Analysis
4.9. Quantitative Phase Imaging
4.10. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Shrestha, A.; Lahooti, B.; Hossian, A.K.M.N.; Madadi, M.; Mikelis, C.M.; Mattheolabakis, G. Stable Dual miR-143 and miR-506 Upregulation Inhibits Proliferation and Cell Cycle Progression. Int. J. Mol. Sci. 2024, 25, 4432. https://doi.org/10.3390/ijms25084432
Shrestha A, Lahooti B, Hossian AKMN, Madadi M, Mikelis CM, Mattheolabakis G. Stable Dual miR-143 and miR-506 Upregulation Inhibits Proliferation and Cell Cycle Progression. International Journal of Molecular Sciences. 2024; 25(8):4432. https://doi.org/10.3390/ijms25084432
Chicago/Turabian StyleShrestha, Archana, Behnaz Lahooti, A. K. M. Nawshad Hossian, Mahboubeh Madadi, Constantinos M. Mikelis, and George Mattheolabakis. 2024. "Stable Dual miR-143 and miR-506 Upregulation Inhibits Proliferation and Cell Cycle Progression" International Journal of Molecular Sciences 25, no. 8: 4432. https://doi.org/10.3390/ijms25084432