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Volume 15
Issue 5
10.3390/d15050663
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Article

Expanding Actinomycetota Diversity in the TBRC Culture Collection through Metabarcoding and Simulated In Situ Cultivation of Thailand’s Mekong River Microbiota

by
Supattra Kitikhun
1,
Paopit Siriarchawattana
1,
Suwanee Chunhametha
1,
Chanwit Suriyachadkun
1,
Pattaraporn Rattanawaree
1,
Chitwadee Phithakrotchanakoon
1,
Piyanun Harnpicharnchai
1,
Lily Eurwilaichitr
2,* and
Supawadee Ingsriswang
1,*
1
Thailand Bioresource Research Center (TBRC), National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand
2
National Energy Technology Center, National Science and Technology Development Agency, Pathum Thani 12120, Thailand
*
Authors to whom correspondence should be addressed.
Diversity 2023, 15(5), 663; https://doi.org/10.3390/d15050663
Submission received: 15 March 2023 / Revised: 8 May 2023 / Accepted: 12 May 2023 / Published: 13 May 2023
(This article belongs to the Special Issue Feature Papers in Microbial Diversity and Culture Collections)
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Abstract

Culture-independent and culture-dependent approaches were employed to investigate the taxonomic diversity and biosynthetic gene cluster potential of Actinomycetota in the Mekong River. Through 16S rRNA gene metabarcoding, 21,103 OTUs were revealed to represent 190 genera and at least 595 species of Actinomycetota, including putatively novel taxa. Conventional and in situ cultivation (IC) methods provided 75 Actinomycetota isolates representing 72 species from 21 genera. Of these, 45 species in 4 genera were new to the Thailand Bioresource Research Center (TBRC), a collection of 20,079 Actinomycetota strains from 660 species. Applying both culture-independent and culture-dependent approaches to the same sample revealed greater diversity among the Actinomycetota in the Mekong River than one approach alone.
Keywords: Actinomycetota; freshwater; in situ cultivation; metabarcoding; culture collection Actinomycetota; freshwater; in situ cultivation; metabarcoding; culture collection

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MDPI and ACS Style

Kitikhun, S.; Siriarchawattana, P.; Chunhametha, S.; Suriyachadkun, C.; Rattanawaree, P.; Phithakrotchanakoon, C.; Harnpicharnchai, P.; Eurwilaichitr, L.; Ingsriswang, S. Expanding Actinomycetota Diversity in the TBRC Culture Collection through Metabarcoding and Simulated In Situ Cultivation of Thailand’s Mekong River Microbiota. Diversity 2023, 15, 663. https://doi.org/10.3390/d15050663

AMA Style

Kitikhun S, Siriarchawattana P, Chunhametha S, Suriyachadkun C, Rattanawaree P, Phithakrotchanakoon C, Harnpicharnchai P, Eurwilaichitr L, Ingsriswang S. Expanding Actinomycetota Diversity in the TBRC Culture Collection through Metabarcoding and Simulated In Situ Cultivation of Thailand’s Mekong River Microbiota. Diversity. 2023; 15(5):663. https://doi.org/10.3390/d15050663

Chicago/Turabian Style

Kitikhun, Supattra, Paopit Siriarchawattana, Suwanee Chunhametha, Chanwit Suriyachadkun, Pattaraporn Rattanawaree, Chitwadee Phithakrotchanakoon, Piyanun Harnpicharnchai, Lily Eurwilaichitr, and Supawadee Ingsriswang. 2023. "Expanding Actinomycetota Diversity in the TBRC Culture Collection through Metabarcoding and Simulated In Situ Cultivation of Thailand’s Mekong River Microbiota" Diversity 15, no. 5: 663. https://doi.org/10.3390/d15050663

APA Style

Kitikhun, S., Siriarchawattana, P., Chunhametha, S., Suriyachadkun, C., Rattanawaree, P., Phithakrotchanakoon, C., Harnpicharnchai, P., Eurwilaichitr, L., & Ingsriswang, S. (2023). Expanding Actinomycetota Diversity in the TBRC Culture Collection through Metabarcoding and Simulated In Situ Cultivation of Thailand’s Mekong River Microbiota. Diversity, 15(5), 663. https://doi.org/10.3390/d15050663

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