Identification of NAC Transcription Factors in Suaeda glauca and Their Responses to Salt Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification and Physicochemical Characterization of NAC Family Members in S. glauca
2.2. Motif, Phylogenetic Tree, and Protein–Protein Interaction Analyses
2.3. Expression Profile Analysis of NAC Genes
2.4. Subcellular Localization
2.5. qRT-PCR Verification
3. Results
3.1. Identification and Physicochemical Properties of the NAC Transcription Factor Family in S. glauca
3.2. Evolutionary Analysis of NAC Families in S. glauca and A. thaliana
3.3. Conserved Motifs of NAC Family Transcription Factors in S. glauca
3.4. Expression Analysis of NAC Genes in S. glauca
3.5. Analysis of NAC Protein–Protein Interaction Network
3.6. qRT-PCR Verification of Transcript Profiles
3.7. Subcellular Localization of SgNAC6 and SgNAC18 Proteins
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer Sequences |
---|---|
SgNAC2 | For-CCGAACTGCAAGTTTTCGAT |
SgNAC2 | Rev-GGGTGTAGCAAATCCCTTGA |
SgNAC14 | For-GCCACCATATCCCTCTTCAA |
SgNAC14 | Rev-CAAATCCCATTTTCACCTTCA |
SgNAC16 | For-TCGGATTGAAGGACCTTTTG |
SgNAC16 | Rev-AGTCCATCAATGGCGGTAAG |
SgNAC25 | For-CGCGATATGGAGTGGTTCTT |
SgNAC25 | Rev-TCATGCATCACCCAATCAGT |
Actin | For-CCGCAAAGATTACATACC |
Actin | Rev-TCACCGAAAGTGCTTCTA |
Name | Number of Amino Acids | Molecular Weight | Theoretical pI | Subcellular Location |
---|---|---|---|---|
SgNAC1 | 476 | 53,159.54 | 5.67 | Extracellular |
SgNAC2 | 476 | 53,734.52 | 5.45 | Nuclear |
SgNAC3 | 436 | 48,661.99 | 4.80 | Nuclear |
SgNAC4 | 417 | 46,272.22 | 5.30 | Nuclear |
SgNAC5 | 416 | 45,552.67 | 4.29 | Extracellular |
SgNAC6 | 363 | 40,803.62 | 6.07 | Nuclear |
SgNAC7 | 334 | 38,096.55 | 8.08 | Nuclear |
SgNAC8 | 317 | 35,238.61 | 4.85 | Nuclear |
SgNAC9 | 315 | 36,363.36 | 6.59 | Nuclear |
SgNAC10 | 302 | 34,461.15 | 7.04 | Nuclear |
SgNAC11 | 285 | 30,799.18 | 4.12 | Extracellular |
SgNAC12 | 264 | 30,666.56 | 8.22 | Nuclear |
SgNAC13 | 263 | 29,969.94 | 9.03 | Nuclear |
SgNAC14 | 126 | 13,603.23 | 6.00 | Nuclear |
SgNAC15 | 245 | 28,377.47 | 6.01 | Nuclear |
SgNAC16 | 243 | 27,807.89 | 7.70 | Nuclear |
SgNAC17 | 229 | 26,021.46 | 9.55 | Nuclear |
SgNAC18 | 211 | 24,039.92 | 5.75 | Nuclear |
SgNAC19 | 209 | 24,255.47 | 9.02 | Nuclear |
SgNAC20 | 203 | 22,706.82 | 4.67 | Extracellular |
SgNAC21 | 103 | 11,972.49 | 5.35 | Nuclear |
SgNAC22 | 143 | 16,120.71 | 4.59 | Extracellular |
SgNAC23 | 133 | 15,816.1 | 9.21 | Nuclear |
SgNAC24 | 132 | 15,558.77 | 10.11 | Nuclear |
SgNAC25 | 130 | 15,335.51 | 8.85 | Nuclear |
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Fu, X.; Zhu, L.; Yu, X.; Yang, Q.; Yuan, F.; Jin, H. Identification of NAC Transcription Factors in Suaeda glauca and Their Responses to Salt Stress. Curr. Issues Mol. Biol. 2024, 46, 8741-8751. https://doi.org/10.3390/cimb46080516
Fu X, Zhu L, Yu X, Yang Q, Yuan F, Jin H. Identification of NAC Transcription Factors in Suaeda glauca and Their Responses to Salt Stress. Current Issues in Molecular Biology. 2024; 46(8):8741-8751. https://doi.org/10.3390/cimb46080516
Chicago/Turabian StyleFu, Xujun, Longmin Zhu, Xiaomin Yu, Qinghua Yang, Fengjie Yuan, and Hangxia Jin. 2024. "Identification of NAC Transcription Factors in Suaeda glauca and Their Responses to Salt Stress" Current Issues in Molecular Biology 46, no. 8: 8741-8751. https://doi.org/10.3390/cimb46080516