Cacalol Acetate as Anticancer Agent: Antiproliferative, Pro-Apoptotic, Cytostatic, and Anti-Migratory Effects
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultures
2.2. Tested Compounds
2.3. Cell Proliferation Assays
2.4. Determination of Apoptosis by Evaluation of Active Caspase-3
2.5. Determination of Autophagic (p62 and LC3B) Biomarkers by Western Blot Analysis
2.6. Determination of Cell Migration
2.7. Determination of Cell Cycle Arrest
2.8. Statistical Analysis
3. Results
3.1. Cytotoxicity Activity of Phytochemical Compounds on HeLa Cells
3.2. Antiproliferative Activity of CA and I3C in HeLa Cells
3.3. Caspase-3-Induced-Apoptosis in HeLa Cells by CA and I3C
3.4. Determination of Autophagy Induced by CA and I3C Treatments in HeLa Cells
3.5. Effect of CA and I3C on Cell Cycle Arrest of HeLa Cells
3.6. Effect of CA and I3C on Cell Migration of HeLa Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rostro-Alonso, G.O.; Castillo-Montoya, A.I.; García-Acosta, J.C.; Aguilar-Llanos, E.F.; Quintas-Granados, L.I.; Villegas-Vazquez, E.Y.; García-Aguilar, R.; Porras-Vázquez, S.A.; Bustamante-Montes, L.P.; Alvarado-Sansininea, J.J.; et al. Cacalol Acetate as Anticancer Agent: Antiproliferative, Pro-Apoptotic, Cytostatic, and Anti-Migratory Effects. Curr. Issues Mol. Biol. 2024, 46, 9298-9311. https://doi.org/10.3390/cimb46090550
Rostro-Alonso GO, Castillo-Montoya AI, García-Acosta JC, Aguilar-Llanos EF, Quintas-Granados LI, Villegas-Vazquez EY, García-Aguilar R, Porras-Vázquez SA, Bustamante-Montes LP, Alvarado-Sansininea JJ, et al. Cacalol Acetate as Anticancer Agent: Antiproliferative, Pro-Apoptotic, Cytostatic, and Anti-Migratory Effects. Current Issues in Molecular Biology. 2024; 46(9):9298-9311. https://doi.org/10.3390/cimb46090550
Chicago/Turabian StyleRostro-Alonso, Gareth Omar, Alejandro Israel Castillo-Montoya, Juan Carlos García-Acosta, Erick Fernando Aguilar-Llanos, Laura Itzel Quintas-Granados, Edgar Yebrán Villegas-Vazquez, Rosario García-Aguilar, Samantha Andrea Porras-Vázquez, Lilia Patricia Bustamante-Montes, Jesús J. Alvarado-Sansininea, and et al. 2024. "Cacalol Acetate as Anticancer Agent: Antiproliferative, Pro-Apoptotic, Cytostatic, and Anti-Migratory Effects" Current Issues in Molecular Biology 46, no. 9: 9298-9311. https://doi.org/10.3390/cimb46090550