Enhancing the Cryopreservation System of Larch Embryogenic Culture by Optimizing Pre-Culture, Osmoprotectants, and Rapid Thawing

Round 1

Reviewer 1 Report

In methods, the it would be interesting to show the explant for cryoprotectant. References for MS need to be included.  Need to clarify the result of TTC in relation to embryogenic cell in liquid medium. See comment in the manuscripts.

Comments for author File: Comments.pdf

The manuscripts need to minorly check in terms of English language

Author Response

Please see the attachment.

Author Response File: Author Response.doc

Reviewer 2 Report

This is an excellent paper describing development of new highly effective protocol for cryopreservation of hybrid larch embryos. The paper is well structured, presented in clear scientific style. Cryopreservation of woody species is extremely important for preserving biodiversity. However, development of efficient protocols is complicated task. The authors reported highly efficient procedure by using  pre-culturing in solid medium, pre-treatment with combination of cryoprotectors and a step with rapid thawing for embryo recovery, which allows them to achieved 80% survival rates of embryogenic tissue. In my opinion, this is important methodological study for cryopreservation of woody species. I have no additional reccomendations.  

 

Author Response

Dear reviewer:

We would also like to express our thanks to the reviewers for your time on our manuscript. We have implemented their comments and suggestions and wish to submit a revised version of the manuscript for further consideration in the journal. Thank you again for your time and effort in this review. Wish everything goes well with your work.

 

Reviewer 3 Report

Dear Authors,

my suggestions can be found in the attached PDF file.

Comments for author File: Comments.pdf

Dear Authors,

my suggestions can be found in the attached PDF file.

Author Response

Please see the attachment

Author Response File: Author Response.docx