Synthesis, Characterization, and Biological Evaluation of Chitosan Nanoparticles Cross-Linked with Phytic Acid and Loaded with Colistin against Extensively Drug-Resistant Bacteria
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Materials
2.2. Chitosan Deacetylation
2.3. Preparation of Chitosan Nanoparticles Cross-Linked with Phytic Acid and Loaded with Colistin
2.4. Physicochemical Characterization
2.4.1. Determination of Colistin Aggregation by Tensiometric Analysis
2.4.2. Determination of Particle Size, Polydispersity Index-PDI, and Zeta Potential of Nanoparticulate Systems
2.4.3. Characterization of Nanoparticulate Systems by DSC and FTIR
2.5. Encapsulation Efficiency
2.6. Antimicrobial Activity
2.7. Statistical Analysis
3. Results and Discussion
3.1. Chitosan Deacetylation
3.2. Preparation of Chitosan Nanoparticles
3.3. Physicochemical Characterization
3.3.1. Determination of Colistin Aggregation by Tensiometric Analysis
3.3.2. Determination of Particle Size, Polydispersity Index-PDI, and Zeta Potential of Nanoparticulate Systems
3.3.3. Characterization of Nanoparticulate Systems by DSC and FTIR
3.4. Encapsulation Efficiency
3.5. Antimicrobial Activity
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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System Dispersed in Water | Physicochemical Parameter | ||
---|---|---|---|
Particle Size (nm) | PDI | (mV) | |
Free chitosan | 2008 ± 155 | 0.670 ± 0.275 | +52.4 ± 1.04 |
Free colistin | 351.8 ± 41.1 | 0.371 ± 0.008 | +5.02 ± 0.65 |
chitosan NPs cross-linked with phytic acid (blank NPs) | 276.5 ± 5.2 | 0.201 ± 0.020 | +33.5 ± 1.4 |
chitosan NPs cross-linked with phytic acid and loaded with colistin (CT-NPs) | 265.8 ± 4.5 | 0.196 ± 0.017 | +42.0 ± 1.7 a |
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Pacheco, F.; Barrera, A.; Ciro, Y.; Polo-Cerón, D.; Salamanca, C.H.; Oñate-Garzón, J. Synthesis, Characterization, and Biological Evaluation of Chitosan Nanoparticles Cross-Linked with Phytic Acid and Loaded with Colistin against Extensively Drug-Resistant Bacteria. Pharmaceutics 2024, 16, 1115. https://doi.org/10.3390/pharmaceutics16091115
Pacheco F, Barrera A, Ciro Y, Polo-Cerón D, Salamanca CH, Oñate-Garzón J. Synthesis, Characterization, and Biological Evaluation of Chitosan Nanoparticles Cross-Linked with Phytic Acid and Loaded with Colistin against Extensively Drug-Resistant Bacteria. Pharmaceutics. 2024; 16(9):1115. https://doi.org/10.3390/pharmaceutics16091115
Chicago/Turabian StylePacheco, Fabian, Alejandro Barrera, Yhors Ciro, Dorian Polo-Cerón, Constain H. Salamanca, and José Oñate-Garzón. 2024. "Synthesis, Characterization, and Biological Evaluation of Chitosan Nanoparticles Cross-Linked with Phytic Acid and Loaded with Colistin against Extensively Drug-Resistant Bacteria" Pharmaceutics 16, no. 9: 1115. https://doi.org/10.3390/pharmaceutics16091115