Deregulated microRNAs Are Associated with Patient Survival and Predicted to Target Genes That Modulate Lung Cancer Signaling Pathways
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Patient Clinical and Histopathological Characteristics
2.2. EGFR and RAS Driver Mutations in Lung AD and SCC
2.3. miRNAs Are Deregulated in Lung AD and SCC Compared to Normal Lung Tissues, and miR-25-3p Overexpression Is Significantly Associated with Poor Survival
2.4. miRNAs Are Predicted to Regulate Genes Abnormally Expressed in Lung AD and SCC, which Modulate Known Pathways of Lung Cancer
3. Discussion
4. Material and Methods
4.1. Ethics Statement
4.2. Patient Samples
4.3. RNA and DNA Extraction
4.4. SNaPShot Assay
4.5. EGFR Exon 19 Deletion Analysis
4.6. EGFR Exon 20 Insertion Analysis
4.7. Quantitative miRNA Expression Analysis by TaqMan Low Density Arrays
4.8. Computational and Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Variables | AD | SCC | p |
---|---|---|---|
Age | |||
Mean (SD) | 60.91 (10.3) | 62 (7.1) | 0.725 |
Range | 40–84 | 51–73 | |
Sex | N (%) | N (%) | |
Male | 13 (56.5) | 11 (73.4) | 0.329 |
Female | 10 (43.5) | 4 (26.6) | |
Smoking | N (%) | N (%) | |
No | 7 (30.5) | 1 (6.7) | 0.114 |
Yes | 16 (69.5) | 14 (93.3) | |
Stage | N (%) | N (%) | |
I | 9 (39.1) | 4 (26.7) | 0.651 |
II | 6 (26.1) | 4 (26.7) | |
III | 8 (34.8) | 6 (40) | |
IV | 0 | 1 (6.6) | |
Death | n (%) | n (%) | |
Cancer-associated | 5 (62.5) | 4 (44.4) | 0.637 |
Other causes | 3 (37.5) | 8 (56.4) |
miRNA | FC | p Value of FC |
---|---|---|
miR-143-3p | 0.326 | <0.001 |
miR-140-5p | 0.369 | 0.049 |
miR-376c-3p | 2.057 | 0.001 |
miR-141-3p | 2.060 | 0.004 |
miR-20a-5p | 2.107 | 0.003 |
miR-199a-3p | 2.199 | 0.003 |
miR-374-5p | 2.260 | <0.001 |
miR-130a-3p | 2.414 | < 0.001 |
miR-29b-3p | 2.511 | <0.001 |
let-7d-5p | 2.580 | <0.001 |
miR-93-5p | 2.751 | <0.001 |
miR-142-3p | 2.920 | 0.007 |
miR-15a-5p | 3.016 | 0.017 |
miR-155-5p | 3.056 | 0.005 |
miR-25-3p * | 3.371 | <0.001 |
miR-429 | 3.593 | <0.001 |
miR-452-5p | 3.668 | 0.007 |
miR-20b-5p | 3.917 | <0.001 |
miR-135b-5p | 4.026 | <0.001 |
miR-708-5p | 4.354 | <0.001 |
miR-200b-3p | 4.446 | <0.001 |
miR-340-5p | 5.345 | <0.001 |
miR-744-5p | 5.649 | <0.001 |
miR-365-3p | 5.766 | <0.001 |
miR-205-5p | 5.856 | 0.001 |
miR-590-5p | 6.112 | 0.001 |
miR-224-5p | 6.692 | <0.001 |
miR-15b-5p | 6.712 | <0.001 |
miR-21-5p | 7.827 | <0.001 |
miR-95-3p | 9.817 | <0.001 |
miR-31-5p | 13.929 | 0.001 |
miR-196b-5p | 16.525 | 0.001 |
miR-411-5p | 25.909 | 0.033 |
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Souza, C.P.; Cinegaglia, N.C.; Felix, T.F.; Evangelista, A.F.; Oliveira, R.A.; Hasimoto, E.N.; Cataneo, D.C.; Cataneo, A.J.M.; Scapulatempo Neto, C.; Viana, C.R.; et al. Deregulated microRNAs Are Associated with Patient Survival and Predicted to Target Genes That Modulate Lung Cancer Signaling Pathways. Cancers 2020, 12, 2711. https://doi.org/10.3390/cancers12092711
Souza CP, Cinegaglia NC, Felix TF, Evangelista AF, Oliveira RA, Hasimoto EN, Cataneo DC, Cataneo AJM, Scapulatempo Neto C, Viana CR, et al. Deregulated microRNAs Are Associated with Patient Survival and Predicted to Target Genes That Modulate Lung Cancer Signaling Pathways. Cancers. 2020; 12(9):2711. https://doi.org/10.3390/cancers12092711
Chicago/Turabian StyleSouza, Cristiano P., Naiara C. Cinegaglia, Tainara F. Felix, Adriane F. Evangelista, Rogério A. Oliveira, Erica N. Hasimoto, Daniele C. Cataneo, Antônio J. M. Cataneo, Cristovam Scapulatempo Neto, Cristiano R. Viana, and et al. 2020. "Deregulated microRNAs Are Associated with Patient Survival and Predicted to Target Genes That Modulate Lung Cancer Signaling Pathways" Cancers 12, no. 9: 2711. https://doi.org/10.3390/cancers12092711