Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patients and Specimens
2.2. Immunohistochemistry
2.3. Immunohistochemical Staining Quantification
2.4. Immunofluorescence
2.5. Proximity Ligation Assay
2.6. Statistical Analysis
2.7. Ethical Statement
3. Results
3.1. Metallothionein I/II Expression in OLP, OL and Different Grades of OSCC
3.2. Megalin Expression in OLP, OL and Different Grades of OSCC
3.3. Co-Expression and Interaction Studies
3.4. Correlation Analysis
3.5. Impact of Smoking, Sex, and Age on MT I/II and Megalin Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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HEALTHY ORAL MUCOSA | ORAL LICHEN PLANUS | ORAL LEUKOPLAKIA | ORAL SQUAMOS CELL CANCER | |||
---|---|---|---|---|---|---|
GRADE I | GRADE II | GRADE III | ||||
N | 10 | 15 | 26 | 28 | 23 | 12 |
SEX (M:F) | 7:3 | 10:5 | 16:10 | 14:14 | 15:8 | 10:2 |
AGE AT DIAGNOSIS | 51.1 ± 15.8 | 63.9 ± 9.8 | 61.4 ± 9.6 | 63.9 ± 8.4 | 65.4 ± 7 | 65.8 ± 10.6 |
SMOKING (Y:N) | 6:4 | 4:11 | 13:13 | 16:12 | 13:10 | 5:7 |
MT—AVERAGE GRAY VALUE: MEAN±SD | 12.0 ± 4.6 | 20.8 ± 4.9 | 28.4 ± 7.6 | 53.7 ± 12.4 | 85.2 ± 23.6 | 106.4 ± 25.4 |
MT—AVERAGE GRAY VALUE: MEDIAN (RANGE) | 11.9 (5.3−18.4) | 19.4 (14.2−31.1) | 27.9 (17.8−46.9) | 53.5 (21.7−93.7) | 83.5 (52.2−137.6) | 114.3 (67.2−133.6) |
MEGALIN—AVERAGE GRAY VALUE: MEAN±SD | 0 | 16.1 ± 8.8 | 25.6 ± 8.5 | 32.8 ± 7.8 | 44.1 ± 12.3 | 81.5 ± 13.4 |
MEGALIN—AVERAGE GRAY VALUE: MEDIAN (RANGE) | 0 | 14.8 (6.1−35.0) | 24.7 (9.6−43.2) | 32.5 (7.1−51.5) | 44.2 (20.4−68.3) | 78.8 (64.6−105.4) |
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Zulijani, A.; Dekanić, A.; Ćabov, T.; Jakovac, H. Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions. Cancers 2021, 13, 4530. https://doi.org/10.3390/cancers13184530
Zulijani A, Dekanić A, Ćabov T, Jakovac H. Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions. Cancers. 2021; 13(18):4530. https://doi.org/10.3390/cancers13184530
Chicago/Turabian StyleZulijani, Ana, Andrea Dekanić, Tomislav Ćabov, and Hrvoje Jakovac. 2021. "Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions" Cancers 13, no. 18: 4530. https://doi.org/10.3390/cancers13184530
APA StyleZulijani, A., Dekanić, A., Ćabov, T., & Jakovac, H. (2021). Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions. Cancers, 13(18), 4530. https://doi.org/10.3390/cancers13184530