High Cell-Free DNA Integrity Is Associated with Poor Breast Cancer Survival
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patients, Sample Material and Clinical Data
2.2. Isolation of Cell-Free DNA
2.3. Concentration and Fragmentation Measurements
2.4. Statistical Analysis
3. Results
3.1. Patient Cohort
3.2. High cfDConc Is Not Associated with Poor BC Survival
3.3. High Cfdi Is An Independent Prognostic Factor for Poor OS and BCSS
3.4. The cfDI Improves the Prediction Accuracy of Logistic Regression Models
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variable | Grouping | Total | ER+ | ER− |
---|---|---|---|---|
Age at diagnosis | ≤39 years | 25 (12.3%) | 15 (10.9%) | 10 (15.2%) |
40–49 years | 47 (23.0%) | 30 (21.7%) | 17 (25.8%) | |
50–59 years | 56 (27.5%) | 36 (26.1%) | 20 (30.3%) | |
60–69 years | 37 (18.1%) | 27 (19.6%) | 10 (15.2%) | |
≥70 years | 39 (19.1%) | 30 (21.7%) | 9 (13.6%) | |
Tumor stage | I | 81 (39.7%) | 59 (42.8%) | 22 (33.3%) |
II | 98 (48.0%) | 66 (47.8%) | 32 (48.5%) | |
II | 25 (12.3%) | 12 (9.4%) | 12 (18.2%) | |
Tumor grade | I | 36 (17.6%) | 34 (24.6%) | 2 (3.0%) |
II | 97 (47.5%) | 78 (56.5%) | 19 (28.8%) | |
II | 70 (43.3%) | 25 (18.1%) | 45 (68.2%) | |
Nodal status | N0 | 115 (56.4%) | 80 (58.0%) | 35 (53.0%) |
N1 | 79 (38.7%) | 53 (38.4%) | 26 (39.4%) | |
N2 | 10 (4.9%) | 5 (3.6%) | 5 (7.6%) | |
PR status | Negative | 95 (46.6%) | 34 (24.6%) | 61 (92.4%) |
Positive | 109 (53.4%) | 104 (75.4%) | 5 (7.6%) | |
HER2 status | Negative | 173 (84.8%) | 122 (88.4%) | 51 (77.3%) |
Positive | 17 (8.3%) | 4 (2.9%) | 13 (19.7%) | |
Missing | 14 (6.9%) | 12 (8.7%) | 2 (3.0%) | |
Chemotherapy | Yes | 43 (21.1%) | 21 (15.2%) | 22 (33.3%) |
No | 161 (78.9%) | 117 (84.8%) | 44 (66.7%) | |
Hormonal therapy | Yes | 51 (25.0%) | 40 (29.0%) | 11 (16.7%) |
No | 153 (75.0%) | 98 (71.0%) | 55 (83.3%) | |
Radiotherapy | Yes | 129 (63.2%) | 86 (62.3%) | 43 (65.2%) |
No | 75 (36.8%) | 52 (37.7%) | 23 (34.8%) |
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Lamminaho, M.; Kujala, J.; Peltonen, H.; Tengström, M.; Kosma, V.-M.; Mannermaa, A. High Cell-Free DNA Integrity Is Associated with Poor Breast Cancer Survival. Cancers 2021, 13, 4679. https://doi.org/10.3390/cancers13184679
Lamminaho M, Kujala J, Peltonen H, Tengström M, Kosma V-M, Mannermaa A. High Cell-Free DNA Integrity Is Associated with Poor Breast Cancer Survival. Cancers. 2021; 13(18):4679. https://doi.org/10.3390/cancers13184679
Chicago/Turabian StyleLamminaho, Maria, Jouni Kujala, Hanna Peltonen, Maria Tengström, Veli-Matti Kosma, and Arto Mannermaa. 2021. "High Cell-Free DNA Integrity Is Associated with Poor Breast Cancer Survival" Cancers 13, no. 18: 4679. https://doi.org/10.3390/cancers13184679