Shifting the Soil: Metformin Treatment Decreases the Protumorigenic Tumor Microenvironment in Epithelial Ovarian Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Tissue Samples
2.2. Desmoplasia Calculation
2.3. Multispectral Spatial Analysis
2.4. Coculture CA-MSC Classification
2.5. In Vivo Mouse MSC/CA-MSC Tumor Xenograft Model
2.6. Statistical Analysis
3. Results
3.1. Clinical Characteristics
3.2. Metformin Decreased the Presence of Carcinoma-Associated Mesenchymal Stem Cells (CA-MSCs)
3.3. Metformin Did Not Impact Desmoplasia
3.4. Metformin Treatment Was Associated with a Reduction in Regulatory T Cells
3.5. Metformin Could Not Reverse CA-MSC Phenotype In Vitro
3.6. Metformin Has the Potential to Prevent the Reprogramming of MSC to a CA-MSC Phenotype
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Taylor, S.E.; Chan, D.K.; Yang, D.; Bruno, T.; Lieberman, R.; Siddiqui, J.; Soong, T.R.; Coffman, L.; Buckanovich, R.J. Shifting the Soil: Metformin Treatment Decreases the Protumorigenic Tumor Microenvironment in Epithelial Ovarian Cancer. Cancers 2022, 14, 2298. https://doi.org/10.3390/cancers14092298
Taylor SE, Chan DK, Yang D, Bruno T, Lieberman R, Siddiqui J, Soong TR, Coffman L, Buckanovich RJ. Shifting the Soil: Metformin Treatment Decreases the Protumorigenic Tumor Microenvironment in Epithelial Ovarian Cancer. Cancers. 2022; 14(9):2298. https://doi.org/10.3390/cancers14092298
Chicago/Turabian StyleTaylor, Sarah E., Daniel K. Chan, Dongli Yang, Tulia Bruno, Richard Lieberman, Javed Siddiqui, Thing Rinda Soong, Lan Coffman, and Ronald J. Buckanovich. 2022. "Shifting the Soil: Metformin Treatment Decreases the Protumorigenic Tumor Microenvironment in Epithelial Ovarian Cancer" Cancers 14, no. 9: 2298. https://doi.org/10.3390/cancers14092298