Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of Gelatin–Hydroxyphenylpropionic Acid
2.2. 1H Nuclear Magnetic Resonance (NMR)
2.3. Electrospinning of Gel–HPA and Gelatin Electrospun Fibers
2.4. Crosslinking of Gel–HPA Fibrous Hydrogels and Gelatin Fibers
2.5. Scanning Electron Microscopy (SEM)
2.6. Water Uptake Measurements
2.7. Mechanical Testing
2.8. Measurement of Enzymatic Degradation
2.9. In Vitro Cell Viability, Spreading and Proliferation
2.10. In Vivo Implantation
2.11. Statistical Analysis
3. Results and Discussion
3.1. Synthesis of Gel–HPA
3.2. The Influence of Crosslinking Parameters on Morphology of Electrospun Scaffolds
3.3. The Physical Properties of Electrospun Scaffolds
3.4. Cell Viability, Spreading and Proliferation on Electrospun Scaffolds
3.5. Histological Staining
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Nie, K.; Han, S.; Yang, J.; Sun, Q.; Wang, X.; Li, X.; Li, Q. Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering. Polymers 2020, 12, 1977. https://doi.org/10.3390/polym12091977
Nie K, Han S, Yang J, Sun Q, Wang X, Li X, Li Q. Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering. Polymers. 2020; 12(9):1977. https://doi.org/10.3390/polym12091977
Chicago/Turabian StyleNie, Kexin, Shanshan Han, Jianmin Yang, Qingqing Sun, Xiaofeng Wang, Xiaomeng Li, and Qian Li. 2020. "Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering" Polymers 12, no. 9: 1977. https://doi.org/10.3390/polym12091977