Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants
Round 1
Reviewer 1 Report
De Carlo et al. have made a comprehensive review on application of temporary immersion system (TIS) for productions of biomass and bioactive compounds from medicinal plants. This review article is well-written with good collection and discussion of published articles on TIS and thus should be of interest to readers. Therefore, I recommend acceptance of this review article after addressing the following comments.
- Keywords should be in full form and so TIS should be expanded.
- A small bibliometric study should be done with the keyword “temporary immersion system” in “Web of Science” for the past 20 years to present the growing trend of its application for biomass and bioactive compounds production from medicinal plants.
- About 2 or 3 figures can be reproduced from reported publications which authors think very important/ground breaking or vital for TIS with respect to biomass and bioactive compounds production. The permission can be sought from the respective publishers for its reproduction and also appropriately acknowledge in the caption.
- The conclusion and perspectives section should be condensed to one or two paragraphs. If two paragraphs, one each can be dedicated for conclusion and perspectives.
Author Response
Please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The presented review "Temporary Immersion System for production of biomass and bioactive compounds from medicinal plants" is a well-structured and written manuscript. The authors present detailed information on the applicability of the temporary immersion technology in plant biotechnology. The manuscript is within the scope of Agronomy. According me the review, it is timely and can be published after minor corrections:
- In tables 1 and 2 "Immersion time (immersion / dry) should be replaced with the correct terminology" Immersion frequency (immersion / stand-by);
- It would be more appropriate to compare the culture systems for biosynthesis of biologically active secondary metabolites on the basis of their productivity and not on the basis of the concentrations of the accumulated metabolites. It matters whether the maximum concentrations accumulate in 2 weeks or in two months! If it is impossible to give this information in the tables, it could be discussed in the text;
- Figure 3 is not readable. Better resolution is needed;
- References 105 should be edited.
Author Response
Please see the attachment
Author Response File: Author Response.pdf
