Pollen: A Potential Explant for Genetic Transformation in Wheat (Triticum aestivum L.)
, Urmil Bansal 1,* and Nabil Ahmad 1,*Round 1
Reviewer 1 Report
1. modify the concluding remarks in abstract.
2. Proper research questions and solid objectives are missing in the study.
3. In methods, appropriate citations of methods used is missing.
4. Please check the lettering used in Tables to show significant difference in mean values.
5. Figures quality can be improved.
6. A solid conclusion based on the study outcomes should be placed before the references section.
Author Response
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Author Response.pdf
Reviewer 2 Report
In this manuscript authors showed the retention of pollen viability in a range of germination media to optimize the viability. Based on scanning electron microscopy, they first examined the pollen morphology of seven genotypes. Then 12 different media were tested on Gladius+Sr50 with different percentages of sucrose and PEG. After that, the percentage of pollen germination was checked at various pH levels, boric concentrations, and calcium nitrate concentrations. Using Impedance Flow Cytometry, they also examined pollen germination in 12 wheat genotypes after 5 hours of harvesting. Overall work is good, but still this work lacks many information like:
1. When testing different sucrose and PEG percentages in germination media, why was Gladius+Sr50 selected?
2. For the morphology study, why were only seven genotypes used?
3. Number of reps used for measuring pollen germination after five hours. This analysis did not include significance calculations.
Author Response
Response attached.
Author Response File: Author Response.pdf
