Nanopriming-Induced Enhancement of Cucumber Seedling Development: Exploring Biochemical and Physiological Effects of Silver Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Green Synthesis of Silver Nanoparticles (AgNPs) and Reaction Mixture Preparation
2.2. Characterization of Silver Nanoparticles
- UV-Visible Spectrophotometry: The UV-visible absorption spectra of the Ag nanoparticle solutions were recorded between 300 and 500 nm using a UV-visible spectrophotometer.
- Transmission Electron Microscopy (TEM): Nanoparticles were suspended in 1 mL of water. A drop of this solution was placed on a carbon-coated copper grid and air-dried. TEM analysis was performed using a JEOL2100 microscope at the Centro Nacional de Microscopía Electrónica, Madrid.
- X-ray Powder Diffraction (XRD): XRD profiles were obtained using an Empyrean Cu LFF X-ray diffractometer within a 2θ range from 2° to 80°. The analyses were conducted at the Research Support Centre (CAI) for X-Ray Diffraction, Universidad Complutense de Madrid. The resulting diffraction patterns were compared with reference patterns from the International Centre for Diffraction Data (ICDD) database.
- Fourier-Transform Infrared Spectroscopy (FTIR): FTIR measurements were carried out using a JASCO (FT/IR-6200) spectrophotometer at the CAI of Physical and Chemical Techniques, Complutense University of Madrid. The interpretation of results followed guidelines provided in infrared spectroscopy tables [14].
2.3. Nanopriming of Cucumber Seeds with AgNPs and In Vitro Cultivation
2.4. In Vitro Cultivation of Cucumber Seedlings
2.5. Measurement of Morphological Parameters during Germination
2.6. Measurement of Photosynthetic Parameters
2.7. Measurement of Biochemical Parameters
2.8. Statistical Analysis
3. Results
3.1. Synthesis and Characterization of Silver Nanoparticles Using Reducing Agents
3.2. Morphological Parameters
3.2.1. No Adverse Effects on Germination Observed with Nanopriming Treatments
3.2.2. Silver Nanoparticle Treatments Enhanced Both Shoot and Root Lengths in Cucumber Seedlings
3.3. Photosynthetic Parameters
3.3.1. Nano-Priming Treatments Significantly Improved the Chlorophyll Index of Cucumber Seedlings
3.3.2. Chlorophyll Fluorescence
3.3.3. Photosynthetic Pigment Content: Chlorophyll and Carotenoids
3.4. Biochemical Parameters
3.4.1. Malondialdehyde Content Varied with Different Nano-Priming Treatments, Indicating Changes in Oxidative Stress Levels
3.4.2. Silver Nanoparticles Induced Significant Changes in Free Amino Acids Content in Cucumber Seedlings
3.4.3. Variation in Total Phenolic Compounds and Flavonoid Contents Observed across Different Nano-Priming Treatments
3.4.4. Nano-Priming Treatments Altered Total Soluble Sugars and Starch Contents in Cucumber Seedlings
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Priming Treatment | Concentration | Germination (%) |
---|---|---|
Water-Control | - | 100 |
Bulk AgNO3 | 100 μM | 0 |
10 mM | 0 | |
GA-AgNPs | 100 μM | 100 |
10 mM | 100 | |
CA-AgNPs | 100 μM | 88.8 ± 9.6 |
10 mM | 100 | |
AA-AgNPs | 100 μM | 83.3 |
10 mM | 100 |
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Pintos, B.; de Diego, H.; Gomez-Garay, A. Nanopriming-Induced Enhancement of Cucumber Seedling Development: Exploring Biochemical and Physiological Effects of Silver Nanoparticles. Agronomy 2024, 14, 1866. https://doi.org/10.3390/agronomy14081866
Pintos B, de Diego H, Gomez-Garay A. Nanopriming-Induced Enhancement of Cucumber Seedling Development: Exploring Biochemical and Physiological Effects of Silver Nanoparticles. Agronomy. 2024; 14(8):1866. https://doi.org/10.3390/agronomy14081866
Chicago/Turabian StylePintos, Beatriz, Hugo de Diego, and Arancha Gomez-Garay. 2024. "Nanopriming-Induced Enhancement of Cucumber Seedling Development: Exploring Biochemical and Physiological Effects of Silver Nanoparticles" Agronomy 14, no. 8: 1866. https://doi.org/10.3390/agronomy14081866