2.1. Site Description
Climate: The field experiments were conducted from 2022 to 2023 on sandy soils at a private farm in the Nubaria area of Beheira Governorate, Egypt, located at 31°56′46.19″ N, 29°44′40.32″ E. The climatic parameters are given in
Figure 1 (FAO, 2014).
Soil properties and water quality: The initial chemical and physical properties of the soil were determined at the beginning of the experiment, and the values are presented in
Table 1. All average values for the chemical and biological properties of the irrigation water (IW) from a well were also measured, as shown in
Table 2.
Type of fish and description of the dimensions of fishponds: The type of fish was tilapia (
Oreochromis niloticus) and they were raised in 10 ponds, and the ponds’ dimensions were 5 m wide × 15 m long × 2 m in depth but the volume of water inside the pond was 112.5 m
3 (5 m width × 15 m length × 1.5 m water depth), and the density of fish was 50 fish per cubic meter; the details are shown in
Figure 2. The water resource of the fish farm is a groundwater well. The water effluent of these fishponds is disposed of when it is necessary to change the water and replace it with fresh water from the groundwater well by withdrawing it with an irrigation pump and conveying it to a sandy depression.
The fish farm under study contains 10 fishponds, and 20% of the total volume of water in these ponds was drained; therefore, the amount of water per day drained = 5 m × 15 m × 1.5 m × 0.2 × 10 ponds = 225 m3 of FWE. The volume of the drained water per year = 225 m3 × 365 day = 82,125 m3/year.
Analysis of the fish farm effluent (FWE) revealed it could supply the soil with significant amounts of plant-available nutrients. The electrical conductivity (EC) of the FWE was measured at 1.94 dS/m, and the acidity (pH) was 7.14. Notably, the FWE contained 350.7 kg of total nitrogen (TN) per year, which represents the sum of all nitrogen forms, both organic (biological) and inorganic (chemical).
The biological nitrogen (bio-N) in the FWE refers to the nitrogen incorporated into organic matter, such as proteins, nucleic acids, and amino acids from living organisms or recently decomposed organic material. In contrast, the chemical nitrogen (chem-N) encompassed all inorganic forms of nitrogen, including nitrate (NO3−), nitrite (NO2−), ammonium (NH4+), and dissolved gaseous nitrogen, e.g., ammonia (NH3).
This total nitrogen content of the FWE was equivalent to applying 1670 kg of ammonium sulfate fertilizer (21%N) annually. Additionally, the FWE provided 799.1 kg of phosphorus and 38.6 kg of potassium per year, demonstrating its potential as a valuable source of essential plant nutrients. It is worth noting that while nitrogen gas (N2) is the most abundant form of nitrogen in the atmosphere, it is relatively unreactive due to the strong triple bond between the two nitrogen atoms. Specialized microorganisms play a crucial role in converting this atmospheric nitrogen gas into more reactive, plant-available forms through the process of nitrogen fixation.
Methane (CH4), on the other hand, is not a form of nitrogen. It is a simple organic molecule composed of one carbon atom and four hydrogen atoms, and it can be a significant greenhouse gas if released into the atmosphere.
The use of this FWE as an alternative to freshwater irrigation has the potential to provide a sustainable and cost-effective way to supply the soil with the necessary nutrients for plant growth, while also reducing reliance on traditional fertilizers.
Biological properties of the fish water effluent: Quantitative measurements of the fungi and bacteria are shown in
Table 3. The total number of bacteria was 16,500 CFU/mL in the fish farm wastewater, while it was 2300 CFU/mL in the irrigation water (IW). The total number of fecal coliform bacteria was 3300 CFU/mL in the fish farm wastewater, while it was 1900 CFU/mL in the irrigation water. The total number of fungi was 520 CFU/mL in the fishpond wastewater, while it was 90 CFU/mL in the irrigation water. The wastewater also contained algae, which, in turn, contained nutrients such as carbon, nitrogen, phosphorus, and potassium, which are important nutrients necessary for the growth and development of microorganisms.
Components of the irrigation system: The irrigation network contained a submersible pump with discharge rate of 45 m3 h−1. The main line (110 mm diameter) was to connect the irrigation water from the groundwater well to the sub-lines (75 mm in diameter). The irrigation water was transferred from the sub-line to the sprinkler line (63 mm in diameter). The design of the experiment included a sprinkler irrigation system with a nozzle diameter of 3/4″, a discharge rate of 1.15 m3/h, a wetting radius of 10 m, and an operating pressure of 2.4 bar.
Fertilization method: Fertilizers for tomato plants were added, namely 150 kg of phosphorous per hectare in the form of superphosphate, and 250 kg of potassium was also added per hectare before planting; these were incorporated into the soil’s surface layers. Moreover, nitrogen fertilizer at a rate of 320 kg nitrogen per hectare was applied in the form of ammonium nitrate and in a water-soluble form at an interval of 7 days through the sprinkler irrigation system using a Venturi injector. The application of N fertilizer started 2 weeks after planting in 12 equal doses, and the application stopped 40 days before the end of the growing season of tomatoes.
Experimental design: An experimental design was conducted for two seasons in 2022 and 2023 with the aim of maximizing the benefits of using fish water effluent (FWE) and evaluating the resource as an effective alternative to traditional irrigation with fresh water (IW). Three replicates of a split-plot design were used to set up the experiment. The main plots included two types of water allocated for irrigation; the first type was fish water effluent (FWE), and the second type was traditional fresh irrigation water (IW). The second factor for the study was the rate of mineral nitrogen fertilization, where four different rates of mineral nitrogen fertilizer (NMF) were added (100%N, 75%N, 50%N, and 25%N), as described as in
Figure 3, which made up the main sub-plots.
Gross irrigation requirements: The total volume of irrigation water for the sprinkler system for tomato production was obtained using Equation (1). The reference evaporation of the crop (ETo) obtained using the daily climate data was used as an input to the modified Penman–Monteith equation [
19]. The tomato crop season has a duration of 155 days and it is divided into the following stages: primary, 30 days; evolutionary, 45 days; middle, 50 days; and the ripening of the fruits, 30 days. The crop coefficient was obtained during the two growing seasons, and they were 0.45, 0.75, 1.15, and 0.80 for the initial, developmental, middle, and late stages, respectively. The total volume of irrigation water during the two growing seasons was 5130 and 5100 m
3 ha
−1/season for season 2022 and 2023, respectively, for the sprinkler irrigation system. The frequency of irrigation was once per day.
where IRg is the irrigation requirement in mm/day, ETo is the reference evapotranspiration in mm/day, Kc is the crop coefficient, I
E is the efficiency of sprinkler irrigation (90%), R is rainfall in mm/day, and LR is the volume of irrigation water required for the leaching process in mm/day.
2.2. Evaluation Parameters
Soil organic matter: The values of organic matter content were measured before planting, during the plants’ growth stages, and after harvesting [
20,
21,
22].
Activity of microorganisms in the root zone: To measure the total population of microorganisms in the root zone, soil samples were collected in three replicates from the rhizosphere of the grown crop in each of the treatment groups before harvest. The count of microorganisms (CFU) in each treatment was carried out at the end of the two tomato growing seasons
Figure 4.
Water application efficiency (WA
E): WA
E is defined as the ratio of actual water storage in the effective root zone to the water applied. WA
E was estimated using Equation (2)
where WA
E is the water application efficiency, Ds is the depth of stored water in the active root zone (mm), and Da is the depth of applied water in the active root zone (mm). Ds was calculated using Equation (3).
where d is the depth of the soil layer (cm), θ1 is the average soil moisture content after irrigation (g/g) in the root zone, θ2 is the average soil moisture content before irrigation (g/g) in the root zone, and ρ is the relative density of the soil (g/cm
3).
Crop production: The productivity of the tomato crop was measured by estimating the productivity of an area of 1 m2 from each experimental plot for each treatment, and the measurement unit for the productivity was kg/m2. The productivity was converted to tons per hectare.
Water productivity of tomato: The water productivity of tomato was calculated according to [
23] as follows
where WP is the water productivity of tomato (kg tomato/m
3 water), Ey is the economic yield of tomato (kg ha
−1), and Ir is the applied volume of the irrigation water (m
3 water ha
−1 per season).
To assess the impact of the experimental design on the microclimate within the cultivated area and its surroundings, a portable weather station was used. Air temperature was measured during the growing period, and Surfer software (version 8) was used to generate heat contour maps.
Statistical analysis: Most of the data’s averages were subjected to statistical analysis in order to test the difference between the different treatments, as described by Snedecor and Cochran [
24]. A joint statistical analysis test was also conducted for the results of the experiment for the years (2022 and 2023) using the method adopted by Steel and Torrie, where, through previous statistical tests, the average values were compared using the least significant differences (LSD) at a significance level of
p < 0.05.