Evaluation of the Reveal® AST (SPECIFIC) for Antimicrobial Susceptibility Testing from Positive Blood Culture Spiked with Carbapenem-Resistant Isolates
Abstract
:1. Introduction
2. Material and Methods
2.1. Bacterial Isolates
2.2. Spiking of Culture Blood Bottles
2.3. Antimicrobial Susceptibility Testing
2.4. The Reveal Technology
2.5. Quality Control (QC)
2.6. Data Analysis
3. Results
3.1. Time to Result
3.2. Analytical Performances
3.3. Results for Carbapenems
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Number of species | 7 | |
Number of antimicrobials | 18 | |
Number of strains | 197 | |
Number of CPE | ||
OXA-48-like | 88 | |
KPC | 10 | |
NDM | 26 | |
VIM | 15 | |
IMP | 5 | |
Multiple carbapenemases | 28 | |
Rare carbapenemases | 5 | |
Number of non-CPE CRE | 20 |
Disk Diffusion | Reveal | |
---|---|---|
Number of total strain–drug pairs | 3348 | 3272 |
Number of S strain–drug pairs | 1051 | 1060 |
Number of I strain–drug pairs | 94 | 127 |
Number of R strain–drug pairs | 2203 | 2085 |
Antimicrobials | Reveal AST Results | Number of | |||||
---|---|---|---|---|---|---|---|
S | I | R | CA % | Very Major Errors (%) | Major Errors (%) | Minor Errors (%) | |
Ampicillin a | 0 | NA | 54 | 100% | 0/37 (0%) | 0/18 (0%) | NA |
Piperacillin | 4 | NA | 192 | 99.5% | 1/194 (0.5%) | 0/3 (0%) | NA |
Piperacillin/Tazobactam | 19 | NA | 173 | 93.2% | 13/186 (7%) | 0/6 (0%) | NA |
Cefoxitin a | 27 | NA | 28 | 85.5% | 8/36 (22%) | 0/19 (0%) | NA |
Cefotaxime | 27 | 0 | 170 | 96.4% | 0/167 (0%) | 3/25 (12%) | 4/30 (13.3%) |
Ceftazidime | 31 | 8 | 157 | 98.0% | 4/161 (2.5%) | 0/31 (0%) | 0/35 (0%) |
Ceftazidime/Avibactam | 127 | NA | 66 | 97.4% | 5/71 (7%) | 0/125 (0%) | NA |
Cefepime | 44 | 19 | 134 | 91.4% | 13/146 (9%) | 1/43 (2.3%) | 3/51 (5.9%) |
Aztreonam | 51 | 7 | 139 | 90.9% | 0/128 (0%) | 11/59 (18.6%) | 7/69 (10.1%) |
Imipenem | 88 | 38 | 71 | 78.7% | 11/71 (15.5%) | 11/106 (10.4%) | 20/126 (15.6%) |
Ertapenem | 27 | NA | 170 | 95.4% | 8/177 (4.5) | 1/20 (5%) | NA |
Meropenem | 109 | 36 | 51 | 87.8% | 10/61 (16.4%) | 1/101 (1%) | 12/136 (8.8%) |
Amikacin | 157 | NA | 40 | 99.0% | 2/42 (4.8%) | 0/155 (0%) | NA |
Gentamicin | 99 | NA | 98 | 99.5% | 0/96 (0%) | 1/100 (1%) | NA |
Tobramycin | 70 | NA | 127 | 97.0% | 4/129 (3.1%) | 2/68 (2.9%) | NA |
Ciprofloxacin | 39 | 12 | 146 | 91.9% | 0/140 (0%) | 6/50 (12%) | 10/57 (17.5%) |
Levofloxacin | 63 | 3 | 131 | 96.9% | 2/132 (1.5%) | 1/61 (1.6%) | 3/64 (4.7%) |
Trimethoprim/ Sulfamethoxazole | 59 | 4 | 134 | 95.9% | 2/135 (1.5%) | 1/60 (1.7%) | 5/62 (8.1%) |
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Girlich, D.; Jousset, A.B.; Emeraud, C.; Rezzoug, I.; Burwell, R.; Singh, P.; Rhodes, P.A.; Naas, T.; Bonnin, R.A.; Dortet, L. Evaluation of the Reveal® AST (SPECIFIC) for Antimicrobial Susceptibility Testing from Positive Blood Culture Spiked with Carbapenem-Resistant Isolates. Pathogens 2024, 13, 722. https://doi.org/10.3390/pathogens13090722
Girlich D, Jousset AB, Emeraud C, Rezzoug I, Burwell R, Singh P, Rhodes PA, Naas T, Bonnin RA, Dortet L. Evaluation of the Reveal® AST (SPECIFIC) for Antimicrobial Susceptibility Testing from Positive Blood Culture Spiked with Carbapenem-Resistant Isolates. Pathogens. 2024; 13(9):722. https://doi.org/10.3390/pathogens13090722
Chicago/Turabian StyleGirlich, Delphine, Agnès B. Jousset, Cécile Emeraud, Inès Rezzoug, Reece Burwell, Pragya Singh, Paul A. Rhodes, Thierry Naas, Rémy A. Bonnin, and Laurent Dortet. 2024. "Evaluation of the Reveal® AST (SPECIFIC) for Antimicrobial Susceptibility Testing from Positive Blood Culture Spiked with Carbapenem-Resistant Isolates" Pathogens 13, no. 9: 722. https://doi.org/10.3390/pathogens13090722