Emergence of Two Different Genotypes of Bagaza Virus (BAGV) Affecting Red-Legged Partridges in Spain, in 2019 and 2021
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Preparation
2.2. Whole-Genome Sequencing and Phylogenetic Analyses
2.3. Polyprotein Analysis
3. Results
3.1. BAGV Isolates
3.2. BAGV Sequences and Phylogenetic Analyses
3.3. Analysis of the Viral Polyproteins
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name of the Isolate | Year | Location | Species | Tissue | RRT-PCR (Ct Value) 1 | Viral Isolation in Vero Cells, (nº of Passages, p) | GenBank Accession nº |
---|---|---|---|---|---|---|---|
BAGV_SPA/E/2019-01/RLP-b/3V | 2019 | Vejer de la Frontera (Cádiz, Spain) | Red-legged partridge | Brain | 11.6 | Positive (2p) | PP236854 |
BAGV_SPA/E/2019-02/RLP-b/3V | 2019 | Vejer de la Frontera (Cádiz, Spain) | Red-legged partridge | Brain | 12.1 | Positive (2p) | PP236853 |
BAGV_SPA/E/2019-03/RLP-b/3V | 2019 | Vejer de la Frontera (Cádiz, Spain) | Red-legged partridge | Brain | 11.9 | Positive (2p) | PP236852 |
BAGV_SPA/E/2021-01/RLP-b/3V | 2021 | Jerez de la Frontera (Cádiz, Spain) | Red-legged partridge | Brain | 12.5 | Positive (3p) | PP236851 |
BAGV-GENOTYPE 2 | BAGV-GENOTYPE 1 | |||||||
---|---|---|---|---|---|---|---|---|
PROTEIN | Amino Acid Position | HQ644143.1 (Spain 2010) Red-Legged Partridge | PP236851 (Spain 2021) Red-Legged Partridge | LC730845.1 (Portugal 2021) Red-Legged Partridge | PP236854 (Spain 2019) Red-Legged Partridge | PP236853 (Spain 2019) Red-Legged Partridge | PP236852 (Spain 2019) Red-Legged Partridge | MF380434.1 (Senegal 2014) Cx. poicilipes |
C | 53 | A | . | . | T | T | T | T |
76 | V | . | . | . | . | . | G | |
83 | K | R | R | . | . | . | ||
92 | M | . | . | . | . | . | . | |
100 | G | S | S | . | . | . | . | |
108 | T | . | . | I | I | I | . | |
109 | L | V | V | T | T | T | S | |
113 | I | . | . | V | V | V | V | |
115 | A | V | V | V | V | V | V | |
116 | V | . | . | A | A | A | A | |
prM | 150 | A | . | . | T | T | T | T |
164 | G | . | . | . | R | . | . | |
166 | I | M | M | M | M | M | M | |
E | 564 | T | . | . | A | A | A | A |
659 | K | . | R | . | . | . | . | |
699 | S | . | F | . | . | . | . | |
NS1 | 838 | E | . | D | . | . | . | . |
841 | E | . | . | G | G | G | G | |
842 | K | . | . | R | R | R | R | |
874 | Q | . | . | . | . | . | P | |
896 | W | L | L | . | . | . | . | |
909 | G | . | . | . | . | . | E | |
1010 | I | . | . | V | V | V | V | |
1051 | K | R | R | . | . | . | . | |
1054 | V | . | . | . | . | . | G | |
NS2A | 1174 | V | M | M | M | M | M | M |
1202 | L | . | M | . | . | . | . | |
NS2B | 1305 | R | . | . | K | K | K | K |
1307 | V | . | . | I | I | I | I | |
1319 | V | . | . | I | I | I | I | |
1332 | I | . | . | V | V | V | . | |
NS3 | 1511 | K | . | . | R | R | R | R |
1547 | H | . | R | . | . | . | . | |
1562 | D | . | . | . | . | . | V | |
1758 | V | . | . | I | I | I | I | |
1834 | M | . | . | V | V | V | V | |
1836 | V | . | . | L | L | L | . | |
1885 | Q | . | P | . | . | . | . | |
1887 | N | . | Y | . | . | . | . | |
2113 | E | D | D | D | D | D | D | |
NS4A | 2215 | V | . | . | A | A | A | . |
2262 | V | . | . | I | I | I | I | |
2265 | T | . | . | A | A | A | A | |
NS4B | 2283 | S | N | N | . | . | . | . |
2288 | A | . | T | . | . | . | . | |
2358 | I | T | T | . | . | . | . | |
2435 | I | V | V | . | . | . | ||
2475 | T | . | . | . | . | . | N | |
2477 | I | . | . | . | . | . | D | |
2478 | E | . | . | . | . | . | R | |
2479 | G | . | . | . | . | . | R | |
2480 | A | . | . | . | . | . | S | |
2481 | A | . | . | . | . | . | S | |
2482 | G | . | . | . | . | . | R | |
2483 | R | . | . | . | . | . | T | |
2484 | I | . | . | . | . | . | D | |
2485 | W | . | . | . | . | . | M | |
2486 | N | . | . | . | . | . | E | |
2487 | A | . | . | . | . | . | C | |
2519 | S | . | . | . | . | . | G | |
NS5 | 2652 | I | V | V | . | . | . | . |
2698 | T | . | . | . | . | . | I | |
2703 | I | V | V | . | . | . | . | |
2799 | N | . | . | K | K | K | K | |
2806 | T | . | . | M | M | M | M | |
2895 | A | . | . | S | S | S | S | |
3048 | G | . | . | S | S | S | . | |
3286 | G | S | S | . | . | . | . | |
3372 | S | . | . | G | G | G | . |
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Aguilera-Sepúlveda, P.; Gómez-Martín, B.; Agüero, M.; Jiménez-Clavero, M.Á.; Fernández-Pinero, J. Emergence of Two Different Genotypes of Bagaza Virus (BAGV) Affecting Red-Legged Partridges in Spain, in 2019 and 2021. Pathogens 2024, 13, 724. https://doi.org/10.3390/pathogens13090724
Aguilera-Sepúlveda P, Gómez-Martín B, Agüero M, Jiménez-Clavero MÁ, Fernández-Pinero J. Emergence of Two Different Genotypes of Bagaza Virus (BAGV) Affecting Red-Legged Partridges in Spain, in 2019 and 2021. Pathogens. 2024; 13(9):724. https://doi.org/10.3390/pathogens13090724
Chicago/Turabian StyleAguilera-Sepúlveda, Pilar, Belén Gómez-Martín, Montserrat Agüero, Miguel Ángel Jiménez-Clavero, and Jovita Fernández-Pinero. 2024. "Emergence of Two Different Genotypes of Bagaza Virus (BAGV) Affecting Red-Legged Partridges in Spain, in 2019 and 2021" Pathogens 13, no. 9: 724. https://doi.org/10.3390/pathogens13090724