A Pathogenic Role for FcγRI in the Immune Response against Chlamydial Respiratory Infection

Round 1

Reviewer 1 Report

Dear  MS Debra Liu

The Manuscript should be accepted with minor English review  

In the present study the authors investigate the hole of the FcyR1 cellular receptor in the pathogenesis of chlamydial respiratory infection. They use a murine model (FcyR1 deficient mice) in order to determine the impact of chlamydial infection on the innate and adaptative immunity of these animals. The study involved histological analysis, immunological and molecular methods. The authors observed that FcyR1 deficient mice showed reduced pulmonary pathology in comparison to infected normal animals. The authors also observed that the deficiency of FcyR1 elicited enhanced Th1 response and reduced M1 macrophage polarization. They conclude that FcyR1 has important hole in inflammatory and respiratory disease and that the knowledge of the mechanisms of FcyR1 iteration with innate and adaptative immunity, should facilitate the development of novel therapeutical drugs.

 

 

Comments for author File: Comments.pdf

Author Response

Thanks to you and the reviewer for the detailed review of our manuscript. We went through the manuscript carefully and revised it for improvement using the “Track Changes” function of MS Word.

1. The ethical statement including the number and the date of approval, and name of the ethics committee was added in the Materials and Methods section of the revised manuscript (lines 68-70).
2. We wrote the "P" value in italic in the  Materials and Methods (line 163) and figure legends (lines 241, 276, 277, 305, 334 and 335) of the revised manuscript.
3. We also checked and revised some other parts of this manuscript, all the modifications were marked using the “Track Changes”.

Reviewer 2 Report

The research article of Zeng et al. described the immune mechanism of FcyRI receptor in mice during the chlamydial infection. In the article, they examined the response of various molecules related to innate and adaptive immune cells by various methods, such as flow cytometry  or quantitative real time PCR.I found no issues in the methodology, statistic or flow of the research, I have only minor comments:

L24 - Gram (capitalised)

L73,73,76, 89, 169, 216,249,253,255,268,281,284,321,326... - please write bacterial names in italics

L82 - please correct the name of HeLa cells (not Hela)

L111 - sterile (not capitalised)

L162 - Please correct 'Šidák's' and not Šídák's

 

Author Response

Thanks for your suggestion, we have revised the manuscript according to your comments using the“Track Changes” function of MS Word.

In the revised manuscript, we modified "gram" as "Gram” (line 24);

the bacterial names was wrote in italics (lines 72, 73, 74, 76, 89,168, 214, 247, 251, 253, 266, 279, 282, 290, 298, 319, 324, and 327);

"Hela" was modified as "HeLa" (line 82);

"Sterile" was modified as "sterile" (line 111);

"Šídák's" was modified as "Šidák's" (line 161).