Enzymatic Stoichiometry Reveals the Metabolic Limitations of Soil Microbes under Nitrogen and Phosphorus Addition in Chinese Fir Plantations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Sites and Experimental Design
2.2. Soil Sampling and Preparation
2.3. Analysis of the Soil’s Physicochemical Properties
2.4. Measurements of Phospholipid Fatty Acids (PLFAs) in the Soil
2.5. Soil Enzymatic Activity Analyses
2.6. Vector Analysis of Resource Limitations of Soil Microbes
2.7. Statistical Analysis
3. Results
3.1. Changes in Soil Properties and Microbial Structure
3.2. Changes in the Activity of Soil Extracellular Enzymes
3.3. Vector-Based Characteristics of Extracellular Enzyme Stoichiometry
3.4. Correlation of Enzymatic Activity and Stoichiometry with the Soil’s Properties and Microbial Structure
4. Discussion
4.1. Effect of N Addition on Soil Enzyme Activity and Stoichiometry
4.2. Effect of P Addition on Soil Enzyme Activity and Stoichiometry
4.3. Implications for Plantation Management
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ren, Y.; Wang, Y.; Zhang, X.; Liu, X.; Liu, P.; Chen, L. Enzymatic Stoichiometry Reveals the Metabolic Limitations of Soil Microbes under Nitrogen and Phosphorus Addition in Chinese Fir Plantations. Microorganisms 2024, 12, 1716. https://doi.org/10.3390/microorganisms12081716
Ren Y, Wang Y, Zhang X, Liu X, Liu P, Chen L. Enzymatic Stoichiometry Reveals the Metabolic Limitations of Soil Microbes under Nitrogen and Phosphorus Addition in Chinese Fir Plantations. Microorganisms. 2024; 12(8):1716. https://doi.org/10.3390/microorganisms12081716
Chicago/Turabian StyleRen, Yan, Ying Wang, Xiulan Zhang, Xionghui Liu, Pei Liu, and Liang Chen. 2024. "Enzymatic Stoichiometry Reveals the Metabolic Limitations of Soil Microbes under Nitrogen and Phosphorus Addition in Chinese Fir Plantations" Microorganisms 12, no. 8: 1716. https://doi.org/10.3390/microorganisms12081716