The Response of Rhizosphere Microbial C and N-Cycling Gene Abundance of Sand-Fixing Shrub to Stand Age Following Desert Restoration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Site Selection and Soil Sampling Preparation
2.3. Determination of Soil Physicochemical Properties
2.4. Shotgun Metagenome Sequencing and Sequence Processing
2.5. Carbon and Nitrogen Functional Gene Screening from Shotgun Metagenome Sequence
2.6. Statistical Analysis
3. Results
3.1. Characteristics of Soil Abiotic and Biotic Factors
3.2. Soil Microbial Community Composition Changes along Stand Age
3.3. Soil Microbial Functional Gene Abundance along Stand Age
3.4. Impacts of Environmental Variables on Rhizosphere Soil Microbial Community Composition and Functional Gene Abundance
4. Discussion
4.1. Effects of Stand Age on Soil Properties
4.2. Effects of Stand Age on the Soil Microbial Community and the Genes Related to C and N Cycling
4.3. Linkages between Rhizosphere Soil Microbial and Environmental Factors
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Properties | Stand Age (Year) | ||||
---|---|---|---|---|---|
11 y | 35 y | 58 y | 66 y | ||
Abiotic factors | TN (g/kg) | 0.23 ± 0.01 b | 0.28 ± 0.03 b | 0.31 ± 0.05 b | 0.57 ± 0.03 a |
TC (g/kg) | 3.86 ± 0.10 c | 4.60 ± 0.14 b | 4.56 ± 0.10 b | 7.08 ± 0.15 a | |
C:N | 16.81 ± 0.70 a | 16.82 ± 1.00 a | 15.57 ± 2.38 a | 12.39 ± 0.63 a | |
AP (mg/kg) | 72.21 ± 0.60 c | 70.64 ± 0.29 c | 78.92 ± 2.29 b | 85.53 ± 0.20 a | |
EC (μS/cm) | 122.37 ± 2.38 c | 148.87 ± 5.63 c | 293.33 ± 2.19 b | 717.33 ± 27.53 a | |
pH | 8.05 ± 0.02 b | 8.11 ± 0.01 a | 7.91 ± 0.01 c | 7.78 ± 0.02 d | |
Biotic factors | MBC (mg/kg) | 49.03 ± 1.18 c | 65.28 ± 0.77 c | 97.80 ± 1.12 b | 164.38 ± 14.63 a |
MBN (mg/kg) | 5.75 ± 0.48 d | 8.54 ± 0.69 c | 13.46 ± 0.69 b | 17.41 ± 0.73 a | |
BG (mg/g/h) | 0.30 ± 0.01 a | 0.12 ± 0.02 c | 0.22 ± 0.04 b | 0.22 ± 0.01 b | |
ALP (μg/g/h) | 35.03 ± 2.73 a | 37.38 ± 5.44 a | 31.10 ± 4.40 a | 37.46 ± 1.70 a | |
NAG (μmol/d/g) | 5.35 ± 1.04 a | 6.09 ± 0.52 a | 6.62 ± 0.73 a | 7.18 ± 1.58 a |
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Li, Y.; Wang, B.; Wang, Z.; He, W.; Wang, Y.; Liu, L.; Yang, H. The Response of Rhizosphere Microbial C and N-Cycling Gene Abundance of Sand-Fixing Shrub to Stand Age Following Desert Restoration. Microorganisms 2024, 12, 1752. https://doi.org/10.3390/microorganisms12091752
Li Y, Wang B, Wang Z, He W, Wang Y, Liu L, Yang H. The Response of Rhizosphere Microbial C and N-Cycling Gene Abundance of Sand-Fixing Shrub to Stand Age Following Desert Restoration. Microorganisms. 2024; 12(9):1752. https://doi.org/10.3390/microorganisms12091752
Chicago/Turabian StyleLi, Yunfei, Bingyao Wang, Zhanjun Wang, Wenqiang He, Yanli Wang, Lichao Liu, and Haotian Yang. 2024. "The Response of Rhizosphere Microbial C and N-Cycling Gene Abundance of Sand-Fixing Shrub to Stand Age Following Desert Restoration" Microorganisms 12, no. 9: 1752. https://doi.org/10.3390/microorganisms12091752