Emergence of NDM-1-Producing Pseudomonas aeruginosa Nosocomial Isolates in Attica Region of Greece
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Collection
2.2. Antimicrobial Susceptibility Testing
2.3. Detection of Antimicrobial Resistance Genes and Known Chromosomal Point Mutations
2.4. Molecular Typing
2.5. Whole-Genome Sequencing (WGS)
2.6. Phylogenomic Relationship
2.7. Nucleotide Accession Numbers
3. Results
3.1. Bacterial Isolates and Details on Patients’ Hospitalization
3.2. Antimicrobial Susceptibility Testing
3.3. Molecular Typing
3.4. Detection of Antimicrobial Resistance Genes and Known Chromosomal Point Mutations
3.5. Genetic Environment of blaNDM-1
3.6. Detection of Virulence Genes
3.7. Phylogenetics
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Patient No. | Age | Sex | Isolate No. | Hospital | Department of Hospitalization | Specimen | Date of Isolation | Days from Hospital Admission to Isolation |
---|---|---|---|---|---|---|---|---|
1 | 73 | M | 9953 | H1 | COVID-19 ICU | Blood | 17 January2022 | 30 |
2 | 63 | F | 9959 | H1 | COVID-19 ICU | BAL | 17 January 2022 | 11 |
3 | 59 | M | 9954 | H1 | COVID-19 ICU | Tissue | 3 February 2022 | 23 |
4 | 67 | F | 9955 | H1 | ICU | Blood | 2 March 2022 | 23 |
5 | 32 | M | 9956 | H1 | ICU | Wound | 8 March 2022 | 220 |
6 | 64 | F | 9957 | H1 | Neurology | Blood | 10 May 2022 | 43 |
7 | 70 | F | 9958 | H1 | Medical | Blood | 17 May 2022 | 14 |
8 | 77 | M | 9951 | H1 | COVID-19 | Urine | 22 May 2022 | 0 |
9 | 51 | F | 9960 | H1 | Neurology | Rectal swab | 7 June 2022 | 116 |
10 | 76 | F | 9962 | H1 | COVID-19 | Drainage | 8 July 2022 | 30 |
11 | 90 | M | 9963 | H1 | Medical | Urine | 22 July 2022 | 0 |
12 | 53 | M | 9964 | H1 | Neurosurgery | BAL | 23 July 2022 | 54 [Transferred from ICU to NS] |
13 | 58 | M | 9965 | H1 | Cardiology | BAL | 27 July 2022 | 0 [Transferred from ICU of another hospital] |
14 | 76 | M | 9966 | H1 | Medical | Urine | 18 August 2022 | 0 |
15 | 89 | F | 9952 | H1 | Medical | Urine | 16 September 2022 | 0 |
16 | 88 | F | 9912 | H2 | Medical | pus | 06 October 2022 | 10 |
17 | 67 | F | 10071 | H2 | Medical | pus | 12 December 2022 | 12 |
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Pappa, O.; Louka, C.; Karadimas, K.; Maikousi, E.; Tzoukmani, A.; Polemis, M.; Panopoulou, A.-D.; Daniil, I.; Chryssou, S.; Mellou, K.; et al. Emergence of NDM-1-Producing Pseudomonas aeruginosa Nosocomial Isolates in Attica Region of Greece. Microorganisms 2024, 12, 1753. https://doi.org/10.3390/microorganisms12091753
Pappa O, Louka C, Karadimas K, Maikousi E, Tzoukmani A, Polemis M, Panopoulou A-D, Daniil I, Chryssou S, Mellou K, et al. Emergence of NDM-1-Producing Pseudomonas aeruginosa Nosocomial Isolates in Attica Region of Greece. Microorganisms. 2024; 12(9):1753. https://doi.org/10.3390/microorganisms12091753
Chicago/Turabian StylePappa, Olga, Christina Louka, Kleon Karadimas, Evangelia Maikousi, Angeliki Tzoukmani, Michalis Polemis, Anna-Danai Panopoulou, Ioannis Daniil, Stella Chryssou, Kassiani Mellou, and et al. 2024. "Emergence of NDM-1-Producing Pseudomonas aeruginosa Nosocomial Isolates in Attica Region of Greece" Microorganisms 12, no. 9: 1753. https://doi.org/10.3390/microorganisms12091753