Shifts in Structure and Assembly Processes of Root Endophytic Community Caused by Climate Warming and Precipitation Increase in Alpine Grassland
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Site
2.2. Experimental Design and Sampling
2.3. DNA Extraction and Gene Sequencing
2.4. Data Statistical Analyses
3. Results
3.1. Diversity and Composition of Root Endophytic Bacterial Community
3.2. Assembly Processes of Root Endophytic Bacterial Community
3.3. Differences in Bacterial Community Composition among Plant Species
3.4. Correlations between Endophytic Bacterial Community Composition and Soil Characteristics
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Plant Species | Treatment | F | P | ||
---|---|---|---|---|---|
Elymus nutans | CK | vs. | W | 1.40 | 0.11 |
CK | vs. | P | 1.40 | 0.14 | |
CK | vs. | WP | 2.75 | 0.03 | |
W | vs. | P | 0.73 | 0.94 | |
W | vs. | WP | 2.89 | 0.02 | |
P | vs. | WP | 2.27 | 0.02 | |
Kobresia humilis | CK | vs. | W | 1.82 | 0.11 |
CK | vs. | P | 1.57 | 0.14 | |
CK | vs. | WP | 1.48 | 0.18 | |
W | vs. | P | 0.81 | 0.72 | |
W | vs. | WP | 2.34 | 0.05 | |
P | vs. | WP | 1.84 | 0.08 | |
Melissilus ruthenicus | CK | vs. | W | 1.54 | 0.09 |
CK | vs. | P | 1.07 | 0.35 | |
CK | vs. | WP | 1.64 | 0.12 | |
W | vs. | P | 1.27 | 0.19 | |
W | vs. | WP | 1.50 | 0.21 | |
P | vs. | WP | 1.38 | 0.08 |
Normalized Stochasticity Ratio | |||
---|---|---|---|
E. nutans | K. humilis | M. ruthenicus | |
SWC/% | 0.80 | 0.40 | 0.20 |
pH | −0.21 | 0.63 | 0.95 |
TC (g/kg) | −1.00 ** | 0.20 | 0.40 |
TN (g/kg) | −1.00 ** | 0.20 | 0.40 |
TP(g/kg) | −0.40 | 0.80 | 1.00 ** |
AP (mg/kg) | −0.40 | 0.00 | 0.60 |
NH4+-N (mg/kg) | −0.80 | 0.40 | 0.80 |
NO3−-N (mg/kg) | −0.40 | 0.00 | 0.60 |
C/N | 0.00 | −0.40 | −0.80 |
N/P | 0.00 | −0.40 | −0.80 |
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Wei, X.; Han, B.; Zhang, J.; Shao, X. Shifts in Structure and Assembly Processes of Root Endophytic Community Caused by Climate Warming and Precipitation Increase in Alpine Grassland. Microorganisms 2024, 12, 1780. https://doi.org/10.3390/microorganisms12091780
Wei X, Han B, Zhang J, Shao X. Shifts in Structure and Assembly Processes of Root Endophytic Community Caused by Climate Warming and Precipitation Increase in Alpine Grassland. Microorganisms. 2024; 12(9):1780. https://doi.org/10.3390/microorganisms12091780
Chicago/Turabian StyleWei, Xiaoting, Bing Han, Jinxin Zhang, and Xinqing Shao. 2024. "Shifts in Structure and Assembly Processes of Root Endophytic Community Caused by Climate Warming and Precipitation Increase in Alpine Grassland" Microorganisms 12, no. 9: 1780. https://doi.org/10.3390/microorganisms12091780