Synthesis of Tellurium Nanoparticles Using Moringa oleifera Extract, and Their Antibacterial and Antibiofilm Effects against Bacterial Pathogens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Biosynthesis of Bio-TeNPs
2.3. Characterization of Bio-TeNPs
2.4. Antibacterial Activity Tests
2.5. Growth Curve Experiments
2.6. Live/Dead Cell Staining
2.7. Morphological Observation of Microorganisms
2.8. Biofilm Formation Inhibition Assay
2.9. Clearance Rate of Bio-TeNPs against E. coli on the Surfaces of Glass Slides
2.10. Cytotoxicity Evaluation of Bio-TeNPs
3. Results
3.1. Characterization of Bio-TeNPs
3.2. Antibacterial Activity of Bio-TeNPs
3.3. Bio-TeNPs Inhibit Bacterial Growth
3.4. LSCM Observation of Bacteria Treated with Bio-TeNPs
3.5. Morphological Changes after Treatment with Bio-TeNPs
3.6. Anti-Biofilm Activity of Bio-TeNPs
3.7. Application of Bio-TeNPs against E. coli on the Surfaces of Glass Slides
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Strains | G+/G− | M. oleifera | Chem-Te(0) | Bio-TeNPs | ||
---|---|---|---|---|---|---|
7 mg/mL | 7 mg/mL | 7 mg/mL | 0.7 mg/mL | 0.07 mg/mL | ||
Shigella dysenteriae CMCC 51252 | G− | 19.0 ± 1.0 | 42.5 ± 2.5 | 53.3 ± 0.5 | 46.0 ± 0.8 | 44.0 ± 0.8 |
Salmonella typhimurium ATCC 14028 | G− | ND | 32.0 ± 0.8 | 43.3 ± 1.3 | 41.3 ± 1.9 | 38.0 ± 2.2 |
Escherichia coli ATCC 25922 | G− | ND | 30.0 ± 1.6 | 48.7 ± 1.9 | 45.3 ± 0.5 | 42.0 ± 1.6 |
Klebsiella pneumoniae ATCC 700603 | G− | 9.3 ± 1.9 | 27.0 ± 1.4 | 43.0 ± 2.1 | 40.3 ± 0.5 | 37.7 ± 0.9 |
Streptococcus pneumoniae ATCC 49619 | G+ | ND | 15.3 ± 0.5 | 29.7 ± 3.1 | 28.7 ± 3.4 | 26.7 ± 0.5 |
Streptococcus agalactiae E442 | G+ | ND | 11.0 ± 0.8 | 26.0 ± 2.2 | 22.0 ± 0.8 | 18.7 ± 0.9 |
Staphylococcus aureus ATCC 25923 | G+ | ND | ND | ND | ND | ND |
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Ao, B.; Jiang, H.; Cai, X.; Liu, D.; Tu, J.; Shi, X.; Wang, Y.; He, F.; Lv, J.; Li, J.; et al. Synthesis of Tellurium Nanoparticles Using Moringa oleifera Extract, and Their Antibacterial and Antibiofilm Effects against Bacterial Pathogens. Microorganisms 2024, 12, 1847. https://doi.org/10.3390/microorganisms12091847
Ao B, Jiang H, Cai X, Liu D, Tu J, Shi X, Wang Y, He F, Lv J, Li J, et al. Synthesis of Tellurium Nanoparticles Using Moringa oleifera Extract, and Their Antibacterial and Antibiofilm Effects against Bacterial Pathogens. Microorganisms. 2024; 12(9):1847. https://doi.org/10.3390/microorganisms12091847
Chicago/Turabian StyleAo, Bo, Honglin Jiang, Xuan Cai, Decheng Liu, Junming Tu, Xiaoshan Shi, Yanxiang Wang, Fei He, Jing Lv, Jingjing Li, and et al. 2024. "Synthesis of Tellurium Nanoparticles Using Moringa oleifera Extract, and Their Antibacterial and Antibiofilm Effects against Bacterial Pathogens" Microorganisms 12, no. 9: 1847. https://doi.org/10.3390/microorganisms12091847