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Microorganisms, Volume 7, Issue 1

2019 January - 26 articles

Cover Story: Numerous reports have already suggested that diverse cis- and trans-acting elements including the promoter are evolutionarily tuned as a complete set for the expression of a nested open reading frame in integrated gene clusters. Here, we propose that the set of innate elements with a naturally overexpressed gene could be considered as a scaffold for an expression system. For a proof-of-principle study, we minimized the mined expression module from metagenomics DNA by truncation and/or serial deletion analysis and constructed a novel expression system by simple substitution of the target gene with the innately overexpressed ORF in this module. We obtained successful expression of several inserts using this simple construct without tuning and codon optimization of the target insert. This strategy could provide a basis to create a catalogue of alternative gene expression systems and supply the extended part of recombinant protein production.

Articles (26)

  • Article
  • Open Access
16 Citations
5,287 Views
15 Pages

Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.

  • Alba Blesa,
  • Mercedes Sánchez,
  • Eva Sacristán-Horcajada,
  • Sandra González-de la Fuente,
  • Ramón Peiró and
  • José Berenguer

A high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these hab...

  • Article
  • Open Access
17 Citations
7,768 Views
16 Pages

Fusarium Species and Mycotoxins Contaminating Veterinary Diets for Dogs and Cats

  • Natalia Witaszak,
  • Łukasz Stępień,
  • Jan Bocianowski and
  • Agnieszka Waśkiewicz

Veterinary diets are intended for diseased animals and may contain cereal grains, mainly maize and/or wheat. These, in turn, are often infected with pathogens of the Fusarium genus, which are able to produce numerous harmful mycotoxins. Forty-two sam...

  • Article
  • Open Access
12 Citations
7,219 Views
10 Pages

A total of 131 contemporary and 33 reference isolates representing a number of multi-locus genotypes of Magnaporthe oryzae were subjected to a PCR test to detect the presence/absence of avirulence (Avr) genes. Results revealed that the more frequentl...

  • Article
  • Open Access
66 Citations
8,394 Views
8 Pages

Palm oil is a valuable crop. This relates to the high economic return from sales of the commodity, where Indonesia is the major producer in the world and the island of Sumatra is the most important region for palm oil production in the country. The i...

  • Article
  • Open Access
6 Citations
4,964 Views
14 Pages

Antibiotics can damage the gut microbiome leading to opportunistic infections and the emergence of antibiotic resistance. Microbiome protection via antibiotic inactivation in the gastrointestinal (GI) tract represents a strategy to limit antibiotic e...

  • Review
  • Open Access
296 Citations
19,770 Views
14 Pages

Nowadays cancer is the second main cause of death in the world. The most known bacterial carcinogen is Helicobacter pylori. Pathogens that can have an impact on cancer development in the gastrointestinal tract are also found in the oral cavity. Some...

  • Review
  • Open Access
422 Citations
27,400 Views
14 Pages

Protein fermentation by gut microbiota contributes significantly to the metabolite pool in the large intestine and may contribute to host amino acid balance. However, we have a limited understanding of the role that proteolytic metabolites have, both...

  • Editorial
  • Open Access
10 Citations
3,848 Views
8 Pages

The incidence of fungal infections has been grossly underestimated in the past decades as a consequence of poor identification techniques and a lack of regular epidemiologic surveys in low- and middle-income countries [...]

  • Article
  • Open Access
116 Citations
13,252 Views
20 Pages

Socioeconomic Status and the Gut Microbiome: A TwinsUK Cohort Study

  • Ruth C. E. Bowyer,
  • Matthew A. Jackson,
  • Caroline I. Le Roy,
  • Mary Ni Lochlainn,
  • Tim D. Spector,
  • Jennifer B. Dowd and
  • Claire J. Steves

Socioeconomic inequalities in health and mortality are well established, but the biological mechanisms underlying these associations are less understood. In parallel, the gut microbiome is emerging as a potentially important determinant of human heal...

  • Article
  • Open Access
13 Citations
4,965 Views
9 Pages

Failure of Staphylococcus aureus to Acquire Direct and Cross Tolerance after Habituation to Cinnamon Essential Oil

  • Xiaoqiu Song,
  • Yue Sun,
  • Qian Zhang,
  • Xiaobo Yang,
  • Feng Zheng,
  • Shoukui He and
  • Yifei Wang

Utilization of sublethal concentrations of cinnamon essential oil (CEO) for food preservation has been proposed. However, exposure to stressful, sublethal growth conditions may induce bacterial tolerance to homologous or heterologous stressing agents...

  • Review
  • Open Access
2,762 Citations
111,997 Views
22 Pages

What is the Healthy Gut Microbiota Composition? A Changing Ecosystem across Age, Environment, Diet, and Diseases

  • Emanuele Rinninella,
  • Pauline Raoul,
  • Marco Cintoni,
  • Francesco Franceschi,
  • Giacinto Abele Donato Miggiano,
  • Antonio Gasbarrini and
  • Maria Cristina Mele

Each individual is provided with a unique gut microbiota profile that plays many specific functions in host nutrient metabolism, maintenance of structural integrity of the gut mucosal barrier, immunomodulation, and protection against pathogens. Gut m...

  • Review
  • Open Access
27 Citations
9,501 Views
16 Pages

Shiga toxin (Stx)-producing Escherichia coli (STEC) infections have become a threat to public health globally because of the severe illnesses that they can trigger, such as hemorrhagic colitis and the post-diarrheal hemolytic uremic syndrome (HUS), c...

  • Article
  • Open Access
8 Citations
5,485 Views
12 Pages

In this study we identified single nucleotide polymorphism (SNP) and sequence characteristic amplification region (SCAR) markers for specific identification of antler-shaped Ganoderma lucidum strains. When the partial mitochondrial SSU rDNA gene sequ...

  • Article
  • Open Access
12 Citations
6,441 Views
14 Pages

Expression and Purification of Chemokine MIP-3α (CCL20) through a Calmodulin-Fusion Protein System

  • Gopal Ramamourthy,
  • Mauricio Arias,
  • Leonard T. Nguyen,
  • Hiroaki Ishida and
  • Hans J. Vogel

Human macrophage inflammatory protein 3α (MIP-3α), also known as CCL20, is a 70 amino acid chemokine that selectively binds and activates chemokine receptor 6 (CCR6). This chemokine is responsible for inducing the migration of immature de...

  • Article
  • Open Access
3 Citations
4,780 Views
11 Pages

Many integrated gene clusters beyond a single genetic element are commonly trapped as the result of promoter traps in (meta)genomic DNA libraries. Generally, a single element, which is mainly the promoter, is deduced from the resulting gene clusters...

  • Article
  • Open Access
45 Citations
9,132 Views
19 Pages

Salinity and Temperature Influence Growth and Pigment Production in the Marine-Derived Fungal Strain Talaromyces albobiverticillius 30548

  • Mekala Venkatachalam,
  • Léa Gérard,
  • Cathie Milhau,
  • Francesco Vinale,
  • Laurent Dufossé and
  • Mireille Fouillaud

Marine-derived fungi that inhabit severe changing environments have gained increasing interest for their ability to produce structurally unique natural products. Fungi belonging to the Talaromyces and the close Penicillium genera are among the most p...

  • Article
  • Open Access
70 Citations
6,923 Views
14 Pages

Exploitation of Three Non-Conventional Yeast Species in the Brewing Process

  • Laura Canonico,
  • Edoardo Galli,
  • Enrico Ciani,
  • Francesca Comitini and
  • Maurizio Ciani

Consumers require high-quality beers with specific enhanced flavor profiles and non-conventional yeasts could represent a large source of bioflavoring diversity to obtain new beer styles. In this work, we investigated the use of three different non-c...

  • Review
  • Open Access
36 Citations
7,682 Views
11 Pages

Aspergillus conida are ubiquitous in the environment, including freshwater, water for bathing, and in drinking water. Vulnerable patients and those suffering from allergic diseases are susceptible to aspergillosis. Avoidance of Aspergillus is of para...

  • Review
  • Open Access
12 Citations
5,440 Views
10 Pages

The discovery and understanding of the mode of action of new antimicrobial agents is extremely urgent, since fungal infections cause 1.5 million deaths annually. Antifungal peptides and proteins represent a significant group of compounds that are abl...

  • Article
  • Open Access
27 Citations
5,498 Views
17 Pages

Employment of L. paracasei K5 as a Novel Potentially Probiotic Freeze-Dried Starter for Feta-Type Cheese Production

  • Antonia Terpou,
  • Ioanna Mantzourani,
  • Alex Galanis,
  • Maria Kanellaki,
  • Eugenia Bezirtzoglou,
  • Argyro Bekatorou,
  • Athanasios A. Koutinas and
  • Stavros Plessas

In the present study, a novel potentially probiotic Lactobacillus paracasei strain, previously isolated from dairy products, was evaluated as a starter culture of Feta-type cheese production. Targeting industrial applications, the starter culture was...

  • Article
  • Open Access
61 Citations
8,800 Views
20 Pages

Seagrasses are vital members of coastal systems, which provide several important ecosystem services such as improvement of water quality, shoreline protection, and serving as shelter, food, and nursery to many species, including economically importan...

  • Communication
  • Open Access
26 Citations
6,497 Views
7 Pages

Susceptibility of Virulent Yersinia pestis Bacteria to Predator Bacteria in the Lungs of Mice

  • Riccardo Russo,
  • Irina Kolesnikova,
  • Thomas Kim,
  • Shilpi Gupta,
  • Androulla Pericleous,
  • Daniel E. Kadouri and
  • Nancy D. Connell

Multi-drug resistant bacterial infections are a serious threat to global public health. Changes in treatment modalities and prudent use of antibiotics can assist in reducing the threat, but new approaches are also required for untreatable cases. The...

  • Review
  • Open Access
11 Citations
5,230 Views
34 Pages

The CAM plasmid-coded isoenzymic diketocamphane monooxygenases induced in Pseudomonas putida ATCC 17453 (NCIMB 10007) by growth of the bacterium on the bicyclic monoterpene (rac)-camphor are notable both for their interesting history, and their strat...

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Microorganisms - ISSN 2076-2607