Antibiofilm Activity of a Trichoderma Metabolite against Xanthomonas campestris pv. campestris, Alone and in Association with a Phage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Growth of Xcc Phages
2.2. Complex φHA6PP
2.3. Isolation of 6-Pentyl-α-pyrone
2.4. Antibiofilm Activity of 6-Pentyl-α-pyrone
2.5. CLSM Analysis for Static Biofilm Evaluation
2.6. CLSM Analysis for Dynamic Biofilm Evaluation
2.7. RNA Extraction and Expression Profiling by qPCR
3. Results
3.1. Antibiofilm Activity of 6-Pentyl-α-pyrone
3.2. CLSM Analysis for Static Biofilm Evaluation
3.3. CLSM Analysis for Dynamic Biofilm Evaluation
3.4. RNA Extraction and Expression Profiling by qPCR
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Papaianni, M.; Ricciardelli, A.; Fulgione, A.; d’Errico, G.; Zoina, A.; Lorito, M.; Woo, S.L.; Vinale, F.; Capparelli, R. Antibiofilm Activity of a Trichoderma Metabolite against Xanthomonas campestris pv. campestris, Alone and in Association with a Phage. Microorganisms 2020, 8, 620. https://doi.org/10.3390/microorganisms8050620
Papaianni M, Ricciardelli A, Fulgione A, d’Errico G, Zoina A, Lorito M, Woo SL, Vinale F, Capparelli R. Antibiofilm Activity of a Trichoderma Metabolite against Xanthomonas campestris pv. campestris, Alone and in Association with a Phage. Microorganisms. 2020; 8(5):620. https://doi.org/10.3390/microorganisms8050620
Chicago/Turabian StylePapaianni, Marina, Annarita Ricciardelli, Andrea Fulgione, Giada d’Errico, Astolfo Zoina, Matteo Lorito, Sheridan L. Woo, Francesco Vinale, and Rosanna Capparelli. 2020. "Antibiofilm Activity of a Trichoderma Metabolite against Xanthomonas campestris pv. campestris, Alone and in Association with a Phage" Microorganisms 8, no. 5: 620. https://doi.org/10.3390/microorganisms8050620