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Open AccessArticle
Diminished Immune Response and Elevated Abundance in Gut Microbe Dubosiella in Mouse Models of Chronic Colitis with GBP5 Deficiency
by
Yichen Li
Yichen Li 1,2,3,*,†
,
Wenxia Wang
Wenxia Wang 2,3,†,
Yuxuan Liu
Yuxuan Liu 4,
Senru Li
Senru Li 4,
Jingyu Wang
Jingyu Wang 4 and
Linlin Hou
Linlin Hou 4,*
1
Medical College, Jiaying University, Meizhou 514031, China
2
Department of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
3
Guangdong Institute of Gastroenterology, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Biomedical Innovation Center, Department of General Surgery, the Six Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China
4
School of Medicine, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomolecules 2024, 14(7), 873; https://doi.org/10.3390/biom14070873 (registering DOI)
Submission received: 5 July 2024
/
Accepted: 19 July 2024
/
Published: 20 July 2024
Abstract
Guanylate binding protein 5 (GBP5) is an emerging immune component that has been increasingly recognized for its involvement in autoimmune diseases, particularly inflammatory bowel disease (IBD). IBD is a complex disease involving inflammation of the gastrointestinal tract. Here, we explored the functional significance of GBP5 using Gbp5 knockout mice and wildtype mice exposed to dextran sulfate sodium (DSS) to generate chronic colitis model. We found that Gbp5 deficiency protected mice from DSS-induced chronic colitis. Transcriptome analysis of colon tissues showed reduced immune responses in Gbp5 knockout mice compared to those in corresponding wildtype mice. We further observed that after repeated DSS exposure, the gut microbiota was altered, both in wildtype mice and Gbp5 knockout mice; however, the gut microbiome health index was higher in the Gbp5 knockout mice. Notably, a probiotic murine commensal bacterium, Dubosiella, was predominantly enriched in these knockout mice. Our findings suggest that GBP5 plays an important role in promoting inflammation and dysbiosis in the intestine, the prevention of which might therefore be worth exploring in regards to IBD treatment.
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MDPI and ACS Style
Li, Y.; Wang, W.; Liu, Y.; Li, S.; Wang, J.; Hou, L.
Diminished Immune Response and Elevated Abundance in Gut Microbe Dubosiella in Mouse Models of Chronic Colitis with GBP5 Deficiency. Biomolecules 2024, 14, 873.
https://doi.org/10.3390/biom14070873
AMA Style
Li Y, Wang W, Liu Y, Li S, Wang J, Hou L.
Diminished Immune Response and Elevated Abundance in Gut Microbe Dubosiella in Mouse Models of Chronic Colitis with GBP5 Deficiency. Biomolecules. 2024; 14(7):873.
https://doi.org/10.3390/biom14070873
Chicago/Turabian Style
Li, Yichen, Wenxia Wang, Yuxuan Liu, Senru Li, Jingyu Wang, and Linlin Hou.
2024. "Diminished Immune Response and Elevated Abundance in Gut Microbe Dubosiella in Mouse Models of Chronic Colitis with GBP5 Deficiency" Biomolecules 14, no. 7: 873.
https://doi.org/10.3390/biom14070873
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