Transcriptome Mechanism of Utilizing Corn Steep Liquor as the Sole Nitrogen Resource for Lipid and DHA Biosynthesis in Marine Oleaginous Protist Aurantiochytrium sp.
Abstract
:1. Introduction
2. Material and Methods
2.1. Materials
2.2. Strain and Culture Conditions
2.3. Biomass Determination
2.4. Lipid and Fatty Acids Analysis
2.5. Sample Collection and Pretreatment
2.6. RNA Isolation
2.7. Library Preparation and Sequencing
2.8. RNA-Seq Data Processing and Transcriptome Analysis
2.9. Quantitative Real-Time Reverse Transcription PCR (qRT-PCR)
2.10. Metabolomic Validation
2.11. Enzymatic Validation
3. Results
3.1. Evaluation of CSL for Lipid and DHA Production by Aurantiochytrium sp.
3.2. Illumina Sequencing, Reads Assembly, and Functional Annotation
3.3. Transcriptome Profiles of Aurantiochytrium sp. Cells under Three-Level CSL Conditions
3.4. DE Genes Related to the CSL Regulation of Growth and Lipid and DHA Synthesis
3.5. Validation of the Transcriptomic Data by qRT-PCR
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Pathways | Gene ID | Description | FPKM | RT-PCR Validation | ||||
---|---|---|---|---|---|---|---|---|
H Group | N Group | L Group | H Group | N Group | L Group | |||
Fatty Acid Synthesis | DN15169 | Polyketide Synthase Subunit A | 66 ± 2.1 | 137.1 ± 3.6 | 211 ± 5.9 | 48.3 ± 2.4 ** | 119 ± 2.7 | 198 ± 3.1 * |
DN16202 | Polyketide Synthase Subunit B | 1.6 ± 0.4 | 3.8 ± 0.6 | 28.9 ± 2.1 | 10.8 ± 3.1 * | 15.7 ± 2.1 | 30.4 ± 2.6 ** | |
DN15010 | Polyketide Synthase Subunit C | 1.6 ± 0.7 | 28.9 ± 2.1 | 32.3 ± 2.4 | 3.2 ± 1.9 ** | 20.4 ± 2.8 ** | 38.4 ± 2.1 | |
DN16221 | Fatty Acid Synthase | 43.7 ± 1.6 | 35.9 ± 1.6 | 29.5 ± 2.1 | 57.2 ± 3.2 ** | 40.2 ± 3.6 | 35.2 ± 4.1 ** | |
DN14594 | Fatty Acid Elongase | 13.4 ± 0.9 | 6.1 ± 0.9 | 3.7 ± 0.6 | ||||
DN5965 | Fatty Acid Desaturase | 9.9 ± 1.2 | 9.4 ± 1.2 | 8.2 ± 0.7 | ||||
Central Carbon Metabolism | DN12414 | Glucose-6-phosphate dehydrogenase | 15.1 ± 0.9 | 54 ± 1.4 | 68.6 ± 2.6 | 10.5 ± 0.9 ** | 43.2 ± 4.2 | 54.2 ± 1.5 * |
DN15488 | Acyl-CoA Synthetase | 44.6 ± 2.5 | 47.8 ± 0.8 | 109.3 ± 2.6 | ||||
DN9564 | Acetyl-CoA Carboxylase | 9.1 ± 2.1 | 20.3 ± 1.4 | 23.6 ± 1.2 | ||||
Nitrogen Metabolism | DN12917 | Glutamate Synthase | 33.7 ± 2.1 | 14.8 ± 1.5 | 12.5 ± 0.5 | |||
DN12820 | Aspartyl Protease | 8.7 ± 0.7 | 7.1 ± 0.9 | 2.4 ± 0.5 | ||||
DN14856 | Asparagine Synthase | 2.5 ± 0.3 | 1 ± 0.5 | 0.8 ± 0.5 | ||||
Signal Transduction | DN11343 | MYB Transcription Factor | 16 ± 0.9 | 19.6 ± 1.8 | 23.5 ± 2.1 | 12.5 ± 2.7 * | 20.5 ± 3.1 | 28.4 ± 1.8 * |
DN15563 | Serine/Threonine Protein Kinase | 10.6 ± 1.4 | 11.1 ± 0.9 | 31.5 ± 2.6 | ||||
DN11433 | Ca2+/Calmodulin-Dependent Protein Kinase | 1.1 ± 0.5 | 9.6 ± 0.7 | 10.5 ± 2.1 | 0.7 ± 0.2 ** | 5.3 ± 0.8 | 12.4 ± 1.7 ** |
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Wang, D.-S.; Yu, X.-J.; Zhu, X.-Y.; Wang, Z.; Li, H.-J.; Wang, Z.-P. Transcriptome Mechanism of Utilizing Corn Steep Liquor as the Sole Nitrogen Resource for Lipid and DHA Biosynthesis in Marine Oleaginous Protist Aurantiochytrium sp. Biomolecules 2019, 9, 695. https://doi.org/10.3390/biom9110695
Wang D-S, Yu X-J, Zhu X-Y, Wang Z, Li H-J, Wang Z-P. Transcriptome Mechanism of Utilizing Corn Steep Liquor as the Sole Nitrogen Resource for Lipid and DHA Biosynthesis in Marine Oleaginous Protist Aurantiochytrium sp. Biomolecules. 2019; 9(11):695. https://doi.org/10.3390/biom9110695
Chicago/Turabian StyleWang, Dong-Sheng, Xin-Jun Yu, Xiao-Yu Zhu, Zhao Wang, Hui-Juan Li, and Zhi-Peng Wang. 2019. "Transcriptome Mechanism of Utilizing Corn Steep Liquor as the Sole Nitrogen Resource for Lipid and DHA Biosynthesis in Marine Oleaginous Protist Aurantiochytrium sp." Biomolecules 9, no. 11: 695. https://doi.org/10.3390/biom9110695