Rice RBH1 Encoding A Pectate Lyase is Critical for Apical Panicle Development
Abstract
:1. Introduction
2. Results
2.1. Identification of rbh1-1 Mutant
2.2. Spikelet Mutation Phenotype of rbh1-1 Mutant
2.3. Gene Cloning and Genetic Complementary Test
2.4. Expression Analysis of RBH1 and Sequence Analysis of RBH1
2.5. The Function of RBH1 Protein
2.6. Subcellular Structure of rbh1-1 Mutant
2.7. The RBH1 Mutation Enhanced the ROS Accumulation
3. Discussion
3.1. Mutation in RBH1 Resulted in Obvious Apical Panicle Defect
3.2. RBH1 Encodes a Pectate Lyase Involved in Pectin Degradation
3.3. The Potential role of ROS in Plant Growth and Panicle Development
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Gene Cloning
4.3. RNA Extraction, RT-PCR and qRT-PCR
4.4. Complementation Test
4.5. In Situ Hybridization
4.6. Immunohistochemical Assay
4.7. Expression, Purification of RBH1 Protein, SDS-PAGE and Enzyme Activity of RBH1 Protein
4.8. ROS Detection
4.9. Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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He, D.; Liang, R.; Long, T.; Yang, Y.; Wu, C. Rice RBH1 Encoding A Pectate Lyase is Critical for Apical Panicle Development. Plants 2021, 10, 271. https://doi.org/10.3390/plants10020271
He D, Liang R, Long T, Yang Y, Wu C. Rice RBH1 Encoding A Pectate Lyase is Critical for Apical Panicle Development. Plants. 2021; 10(2):271. https://doi.org/10.3390/plants10020271
Chicago/Turabian StyleHe, Dong, Rui Liang, Tuan Long, Ying Yang, and Changyin Wu. 2021. "Rice RBH1 Encoding A Pectate Lyase is Critical for Apical Panicle Development" Plants 10, no. 2: 271. https://doi.org/10.3390/plants10020271