Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves
Abstract
:1. Introduction
2. Results
2.1. An Arabidopsis OLE1-GFP Transgenic Line with a T-DNA Insertion in GBSS1 Accumulated Significant Leaf TAG
2.2. Overexpression of OLE1 in a gbss1 Background Resulted in Higher TAG Accumulation Than in WT
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Genetic Constructs
4.3. Preparation of Leaf Starch Granules and Iodine Staining
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhai, Z.; Liu, H.; Shanklin, J. Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves. Plants 2021, 10, 513. https://doi.org/10.3390/plants10030513
Zhai Z, Liu H, Shanklin J. Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves. Plants. 2021; 10(3):513. https://doi.org/10.3390/plants10030513
Chicago/Turabian StyleZhai, Zhiyang, Hui Liu, and John Shanklin. 2021. "Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves" Plants 10, no. 3: 513. https://doi.org/10.3390/plants10030513