A Leaf Disc Assay for Evaluating the Response of Tea (Camellia sinensis) to PEG-Induced Osmotic Stress and Protective Effects of Azoxystrobin against Drought
Abstract
:1. Introduction
2. Results
2.1. Chlorophyll Fluorescence in Leaf Discs Collected from PEG-Induced Osmotic Stress
2.2. Photosynthetic Pigments in Leaf Discs Collected from PEG-Induced Osmotic Stress
2.3. Antioxidant Status in Leaf Discs Collected from PEG-Induced Osmotic Stress
2.4. Chlorophyll Fluorescence in Leaf Discs of Exogenous AZ Experiment
2.5. Photosynthetic Pigments in Leaf Discs of Exogenous AZ Experiment
2.6. Antioxidant Status in Leaf Discs of Exogenous AZ Experiment
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Measurements of Chlorophyll Fluorescence
4.3. Determination of Photosynthetic Pigments
4.4. Estimation of Total Phenols
4.5. Antioxidant Capacity Assays
4.6. Determination of Lipid Peroxidation
4.7. Measurement of Ascorbate Peroxidase Activity
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Effect | Regression Coefficient | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Fv/Fm | Y(II) | Y(NO) | Y(NPQ) | Total Chl | Chl a/b | Car | DPPH Scavenging | Reducing Power | Total Phenols | MDA | |
Intercept | 0.740 *** | 0.223 *** | 0.229 *** | 0.538 *** | 11.513 *** | 2.009 *** | 2.008 *** | 69.911 *** | 281.493 *** | 112.184 *** | 0.285 *** |
d | 0.059 ** | 0.051 *** | 0.137 *** | −0.204 *** | 4.468 *** | 0.097 *** | 0.494 *** | 50.697 *** | 163.475 *** | 42.046 *** | −0.054 *** |
D2 | 0.033 ** | - | 0.053 *** | −0.069 *** | 1.848 *** | 0.077*** | 0.229 *** | 20.964 *** | 66.495 *** | 18.553 *** | - |
t | −0.008 * | −0.023 *** | 0.067 *** | −0.033 * | −0.452 *** | 0.005 NS | 0.098 ** | 5.409 * | 8.513 NS | 7.081 ** | 0.044 *** |
t2 | - | - | −0.009 *** | 0.007 ** | - | - | −0.023 *** | −1.173 ** | −2.238 ** | −1.241 ** | −0.006 *** |
dt | 0.051 *** | - | −0.069 *** | 0.063 *** | 0.456 *** | 0.047 *** | 0.108 *** | 4.390 *** | 8.642 *** | 5.547 *** | - |
Root MSE | 0.037 | 0.033 | 0.051 | 0.052 | 0.613 | 0.034 | 0.119 | 6.815 | 14.879 | 7.774 | 0.028 |
R2 | 0.936 | 0.715 | 0.914 | 0.828 | 0.940 | 0.902 | 0.909 | 0.915 | 0.942 | 0.883 | 0.743 |
Adj-R2 | 0.933 | 0.710 | 0.910 | 0.819 | 0.937 | 0.897 | 0.904 | 0.909 | 0.938 | 0.876 | 0.733 |
AZ Conc. (g L−1) | Treatment | Total Chl (mg g−1 DW) | Chl a/b (mg mg−1) | Car (mg g−1 DW) |
---|---|---|---|---|
No AZ | Pre-treat | 9.87 ± 0.43 a | 1.883 ± 0.005 cd | 1.71 ± 0.07 cd |
Blank | 9.90 ± 0.38 a | 1.912 ± 0.011 ab | 1.95 ± 0.07 a | |
PEG | 8.90 ± 0.35 cd | 1.807 ± 0.016 f | 1.97 ± 0.07 a | |
0.125 | Pre-treat | 9.45 ± 0.41 ab | 1.880 ± 0.012 de | 1.63 ± 0.05 d |
Blank | 9.80 ± 0.31 a | 1.926 ± 0.007 a | 1.85 ± 0.08 b | |
PEG | 8.43 ± 0.25 d | 1.790 ± 0.020 f | 1.68 ± 0.03 cd | |
1.25 | Pre-treat | 8.84 ± 0.38 cd | 1.861 ± 0.004 e | 1.54 ± 0.07 e |
Blank | 9.16 ± 0.47 bc | 1.900 ± 0.011 bc | 1.74 ± 0.08 c | |
PEG | 7.60 ± 0.21 e | 1.761 ± 0.021 g | 1.51 ± 0.05 e |
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Chiu, Y.-C.; Chen, B.-J.; Su, Y.-S.; Huang, W.-D.; Chen, C.-C. A Leaf Disc Assay for Evaluating the Response of Tea (Camellia sinensis) to PEG-Induced Osmotic Stress and Protective Effects of Azoxystrobin against Drought. Plants 2021, 10, 546. https://doi.org/10.3390/plants10030546
Chiu Y-C, Chen B-J, Su Y-S, Huang W-D, Chen C-C. A Leaf Disc Assay for Evaluating the Response of Tea (Camellia sinensis) to PEG-Induced Osmotic Stress and Protective Effects of Azoxystrobin against Drought. Plants. 2021; 10(3):546. https://doi.org/10.3390/plants10030546
Chicago/Turabian StyleChiu, Yu-Chieh, Bo-Jen Chen, Yen-Shuo Su, Wen-Dar Huang, and Chang-Chang Chen. 2021. "A Leaf Disc Assay for Evaluating the Response of Tea (Camellia sinensis) to PEG-Induced Osmotic Stress and Protective Effects of Azoxystrobin against Drought" Plants 10, no. 3: 546. https://doi.org/10.3390/plants10030546