Genome-Wide Analysis of Tubulin Gene Family in Cassava and Expression of Family Member FtsZ2-1 during Various Stress
Abstract
:1. Introduction
2. Results
2.1. Identification of the Tubulin Proteins in Cassava
2.2. Multiple Sequence Alignment, Phylogenetic Analysis, and Classification of MeTubulins
2.3. Gene Structure and Motif Composition of MeTubulins
2.4. Chromosome Distribution and Synteny Analysis of MeTubulins Gene
2.5. Expression Profiles of MeTubulins in Different Tissue Types
2.6. Expression Patterns of FtsZ2-1 Gene in Response to Various Stresses
3. Discussion
4. Materials and Methods
4.1. Identification of the Tubulin Gene Family
4.2. Sequence Analysis and Structural Characterization
4.3. Chromosomal Localization and Gene Duplication
4.4. Phylogenetic Analysis of Cassava Tubulin Genes Family
4.5. RNA-Seq Data Analysis of Tubulin Genes
4.6. Plant Materials and Treatments
4.7. RNA Extraction and qRT-PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | Gene ID | p-Value | Size (aa) | MW (KDa) | pI | Predicted Subcellular Location | Chr. |
---|---|---|---|---|---|---|---|
MeTubulin1 | Manes.08G061700 | 5.6 × 10−114 | 454 | 50.31 | 4.76 | cytoplasm | 8 |
MeTubulin2 | Manes.08G024800 | 2.6 × 10−43 | 492 | 51.13 | 5.56 | chloroplast thylakoid membrane | 8 |
MeTubulin3 | Manes.02G136200 | 3.4 × 10−114 | 453 | 50.06 | 4.77 | cytoplasm | 2 |
MeTubulin4 | Manes.02G123600 | 2.8 × 10−114 | 454 | 50.35 | 4.75 | cytoplasm | 2 |
MeTubulin5 | Manes.05G200200 | 2.7 × 10−114 | 453 | 50.08 | 4.77 | cytoplasm | 5 |
MeTubulin6 | Manes.05G025900 | 6 × 10−94 | 481 | 53.40 | 5.70 | cytoplasm | 5 |
MeTubulin7 | Manes.05G147000 | 1.5 × 10−112 | 456 | 50.38 | 4.74 | cytoplasm | 5 |
MeTubulin8 | Manes.13G106300 | 8.6 × 10−94 | 424 | 45.79 | 4.93 | cytoplasm | 13 |
MeTubulin9 | Manes.09G100400 | 2.2 × 10−113 | 451 | 49.93 | 4.85 | cytoplasm | 9 |
MeTubulin10 | Manes.09G055800 | 1.6 × 10−42 | 493 | 51.31 | 5.96 | chloroplast stroma | 9 |
MeTubulin11 | Manes.09G140200 | 1.5 × 10−98 | 456 | 49.69 | 5.00 | cytoplasm | 9 |
MeTubulin12 | Manes.06G058600 | 8 × 10−112 | 451 | 49.89 | 4.78 | cytoplasm | 6 |
MeTubulin13 | Manes.06G007200 | 2 × 10−114 | 454 | 50.39 | 4.72 | cytoplasm | 6 |
MeTubulin14 | Manes.06G147900 | 9 × 10−115 | 454 | 50.33 | 4.75 | cytoplasm | 6 |
MeTubulin15 | Manes.15G108500 | 3.3 × 10−99 | 456 | 49.70 | 5.00 | cytoplasm | 15 |
MeTubulin16 | Manes.01G249000 | 7.2 × 10−94 | 480 | 53.24 | 5.67 | cytoplasm | 1 |
MeTubulin17 | Manes.01G061400 | 4.2 × 10−112 | 453 | 50.12 | 4.68 | cytoplasm | 1 |
MeTubulin18 | Manes.01G166100 | 2.6 × 10−114 | 454 | 50.35 | 4.74 | cytoplasm | 1 |
MeTubulin19 | Manes.14G124600 | 1.1 × 10−113 | 451 | 49.84 | 4.78 | cytoplasm | 14 |
MeTubulin20 | Manes.03G135500 | 6.1 × 10−38 | 421 | 43.29 | 6.93 | chloroplast stroma | 3 |
MeTubulin21 | Manes.03G098100 | 2.8 × 10−100 | 457 | 49.74 | 4.90 | cytoplasm | 3 |
MeTubulin22 | Manes.10G087300 | 3.7 × 10−100 | 457 | 49.57 | 4.93 | cytoplasm | 10 |
MeTubulin23 | Manes.10G087200 | 3.8 × 10−73 | 421 | 45.61 | 4.74 | cytoplasm | 10 |
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Li, S.; Cao, P.; Wang, C.; Guo, J.; Zang, Y.; Wu, K.; Ran, F.; Liu, L.; Wang, D.; Min, Y. Genome-Wide Analysis of Tubulin Gene Family in Cassava and Expression of Family Member FtsZ2-1 during Various Stress. Plants 2021, 10, 668. https://doi.org/10.3390/plants10040668
Li S, Cao P, Wang C, Guo J, Zang Y, Wu K, Ran F, Liu L, Wang D, Min Y. Genome-Wide Analysis of Tubulin Gene Family in Cassava and Expression of Family Member FtsZ2-1 during Various Stress. Plants. 2021; 10(4):668. https://doi.org/10.3390/plants10040668
Chicago/Turabian StyleLi, Shuangbao, Peng Cao, Congcong Wang, Jianchun Guo, Yuwei Zang, Kunlin Wu, Fangfang Ran, Liangwang Liu, Dayong Wang, and Yi Min. 2021. "Genome-Wide Analysis of Tubulin Gene Family in Cassava and Expression of Family Member FtsZ2-1 during Various Stress" Plants 10, no. 4: 668. https://doi.org/10.3390/plants10040668