Anti-Neuroinflammatory Effects and Mechanism of Action of Fructus ligustri lucidi Extract in BV2 Microglia
Abstract
:1. Introduction
2. Results
2.1. Effects of Fructus Ligustri Lucidi Extract (FLLE) on Inflammatory Response Induced by Lipopolysaccharide (LPS) Stimulation in BV2 Cells
2.2. Effects of FLLE on M1 Microglia Polarization by LPS Stimulation in BV2 Cells
2.3. Effects of FLLE on Inflammatory Mitogen-Activated Protein Kinases (MAPK) and Nuclear Factor-Kappa B (NF-κB) Signaling Pathways in BV2 Cells
2.4. Effects of FLLE on Heme Oxygenase (HO)-1 Expression in BV2 Cells
2.5. Effects of FLLE on the Migratory Ability of BV2 Cells
2.6. Quality Control of FLLE
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Preparation of FLLE
4.3. High-Performance Liquid Chromatography (HPLC)
4.4. Cell Culture and Drug Treatment
4.5. Cell Viability Assay
4.6. Nitric Oxide Production Assay
4.7. Enzyme-Linked Immunosorbent Assay (ELISA)
4.8. Cell Migration Assay
4.9. Western Blotting
4.10. Immunofluorescence Microscopy
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, Y.J.; Park, S.Y.; Koh, Y.J.; Lee, J.-H. Anti-Neuroinflammatory Effects and Mechanism of Action of Fructus ligustri lucidi Extract in BV2 Microglia. Plants 2021, 10, 688. https://doi.org/10.3390/plants10040688
Kim YJ, Park SY, Koh YJ, Lee J-H. Anti-Neuroinflammatory Effects and Mechanism of Action of Fructus ligustri lucidi Extract in BV2 Microglia. Plants. 2021; 10(4):688. https://doi.org/10.3390/plants10040688
Chicago/Turabian StyleKim, Yeon Ju, Sung Yun Park, Young Jun Koh, and Ju-Hee Lee. 2021. "Anti-Neuroinflammatory Effects and Mechanism of Action of Fructus ligustri lucidi Extract in BV2 Microglia" Plants 10, no. 4: 688. https://doi.org/10.3390/plants10040688