Metabolic Analysis of Root, Stem, and Leaf of Scutellaria baicalensis Plantlets Treated with Different LED Lights
Abstract
:1. Introduction
2. Results
2.1. HPLC Analysis of Baicalin, Baicalein, and Wogonin in Root, Stem, and Leaf of S. baicalensis Treated with Different LED Light Sources
2.2. Metabolite-Specific Profiling of Root, Stem, and Leaf of S. baicalensis Treated with Different LED Light Sources
3. Discussion
4. Materials and Methods
4.1. Preparation of Plant Materials
4.2. High-Performance Liquid Chromatography (HPLC) Analysis for Flavones
4.3. Gas Chromatography Time-of-Flight Mass Spectrometry (GC-TOFMS) Analysis
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Appendix A
LED Plant Growth Chamber | |
---|---|
Product | Multi-Room Chamber HB-302S-4 (Hanbaek Scientific Co.,) |
Picture, which is taken from a previous study [10] | |
Dimension of each room (L × W × H) | 136 cm × 78 cm × 168 cm |
LED lights | The white (450–660 nm), blue (450 nm), or red (660 nm) LED lights (PGL-PFL series) were manufactured from PARUS LED Co., Cheonan, Korea |
HPLC Analysis Performed Using Our Previous Study [25] | |
---|---|
Equipment | NS-4000 HPLC apparatus (Futecs, Daejeon, Korea) |
Detector | UV-Vis |
Column | optimapak C18 column (250 mm × 4.6 mm, 5 µm; RStech, Daejon, Korea) |
Detector wavelength | 275 nm |
Oven temperature | 30 °C |
Flow rate | 1.0 mL/min |
Mobile phase | Acetonitrile, solvent A and 0.2% (v/v) acetic acid, solvent B |
Gradient program | Solvent B 90%; 0 min, solvent B 80%; 10 min, solvent B 80%; 15 min, solvent B 75%; 20 min, solvent B 75%; 25 min, solvent B 40%; 50 min, solvent B 90%; 50.1–60 min |
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Yeo, H.-J.; Park, C.-H.; Park, S.-Y.; Chung, S.-O.; Kim, J.-K.; Park, S.-U. Metabolic Analysis of Root, Stem, and Leaf of Scutellaria baicalensis Plantlets Treated with Different LED Lights. Plants 2021, 10, 940. https://doi.org/10.3390/plants10050940
Yeo H-J, Park C-H, Park S-Y, Chung S-O, Kim J-K, Park S-U. Metabolic Analysis of Root, Stem, and Leaf of Scutellaria baicalensis Plantlets Treated with Different LED Lights. Plants. 2021; 10(5):940. https://doi.org/10.3390/plants10050940
Chicago/Turabian StyleYeo, Hyeon-Ji, Chang-Ha Park, Soo-Yun Park, Sun-Ok Chung, Jae-Kwang Kim, and Sang-Un Park. 2021. "Metabolic Analysis of Root, Stem, and Leaf of Scutellaria baicalensis Plantlets Treated with Different LED Lights" Plants 10, no. 5: 940. https://doi.org/10.3390/plants10050940