Exogenous Methyl Jasmonate Improves Cold Tolerance with Parallel Induction of Two Cold-Regulated (COR) Genes Expression in Triticum aestivum L.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material, Growth Conditions, and Treatments
2.2. Determination of Cold Tolerance
2.3. Gas Exchange and Chlorophyll a Fluorescence Measurements
2.4. Determination of H2O2
2.5. Determination of Lipid Peroxidation
2.6. Analysis of Antioxidant Enzyme Activities
2.7. Determination of Free Proline Content
2.8. Gene Expression
2.9. Statistical Analysis
3. Results
3.1. Effect of MJ on Growth and Biomass Accumulation of Wheat Seedlings
3.2. Exogenous MJ Improves Cold Tolerance of Wheat Seedlings
3.3. Exogenous MJ Stabilizes Wheat Photosynthesis
3.4. Impact of Exogenous MJ on the H2O2 and MDA Content in Wheat
3.5. Exogenous MJ Enhances Activity of Antioxidant Enzymes and Expression of Their Genes in Wheat Leaves
3.6. Exogenous MJ Enhances Free Proline Content
3.7. Effect of Exogenous MJ on COR Genes Expression in Wheat
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Exposure | Shoot DW (mg) | Root DW (mg) |
---|---|---|
22 °C | 26.7 ± 0.1 a | 7.9 ± 0.3 b |
22 °C + MJ | 27.7 ± 0.1 a | 8.8 ± 0.5 a |
4 °C | 19.9 ± 0.5 c | 6.3 ± 0.1 c |
4 °C + MJ | 21.4 ± 0.5 b | 7.2 ± 0.4 b |
Treatment | Time (d) | |||
---|---|---|---|---|
0 | 1 | 3 | 7 | |
4 °C | −5.6 ± 0.03 g | −6.8 ± 0.09 e | −7.9 ± 0.08 c | −8.6 ± 0.05 b |
4 °C + MJ | −5.9 ± 0.06 f | −7.3 ± 0.04 d | −8.4 ± 0.07 b | −9.1 ± 0.10 a |
Time (d) | H2O2 Content (μmol g−1 FW) | MDA Content (nmol g−1 FW) | ||
---|---|---|---|---|
4 °C | 4 °C + MJ | 4 °C | 4 °C + MJ | |
0 | 56.8 ± 1.6 b | 51.6 ± 3.7 bc | 23.3 ± 0.4 d | 25.7 ± 0.8 c |
1 | 64.3 ± 2.3 a | 49.3 ± 1.8 c | 31.9 ± 1.4 a | 26.6 ± 1.7 b |
3 | 59.5 ± 3.1 ab | 54.9 ± 2.6 bc | 30.4 ± 1.5 ab | 27.7 ± 0.7 b |
7 | 47.8 ± 3.3 c | 50.4 ± 3.6 bc | 30.3 ± 0.5 a | 27.9 ± 0.6 b |
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Repkina, N.; Ignatenko, A.; Holoptseva, E.; MiszalskI, Z.; Kaszycki, P.; Talanova, V. Exogenous Methyl Jasmonate Improves Cold Tolerance with Parallel Induction of Two Cold-Regulated (COR) Genes Expression in Triticum aestivum L. Plants 2021, 10, 1421. https://doi.org/10.3390/plants10071421
Repkina N, Ignatenko A, Holoptseva E, MiszalskI Z, Kaszycki P, Talanova V. Exogenous Methyl Jasmonate Improves Cold Tolerance with Parallel Induction of Two Cold-Regulated (COR) Genes Expression in Triticum aestivum L. Plants. 2021; 10(7):1421. https://doi.org/10.3390/plants10071421
Chicago/Turabian StyleRepkina, Natalia, Anna Ignatenko, Ekaterina Holoptseva, Zbigniew MiszalskI, Paweł Kaszycki, and Vera Talanova. 2021. "Exogenous Methyl Jasmonate Improves Cold Tolerance with Parallel Induction of Two Cold-Regulated (COR) Genes Expression in Triticum aestivum L." Plants 10, no. 7: 1421. https://doi.org/10.3390/plants10071421