Enhanced Synthesis of Foreign Nuclear Protein Stimulates Viral Reproduction via the Induction of γ-Thionin Expression
Abstract
:1. Introduction
2. Results
2.1. Massive Synthesis of a Model NLS-Containing Protein Stimulates Reproduction of a crTMV-Based Viral Vector
2.2. NbγThio Expression Is Activated in Response to a Model NLS-Containing Protein
2.3. The NbγThio Promoter (PrγThio) Is Sensitive to the Intensive Accumulation of the Model Nuclear Protein
2.4. Co-Expression of crTMV:GFP Vector with 35S-NbγThio Resulted in the Increased Accumulation of Viral RNA and Enhanced GFP Production
3. Discussion
4. Materials and Methods
4.1. Plant Growth Conditions
4.2. Plasmid Constructs
4.3. Isolation of the γ-Thionin Promoter Region (PrγThio)
4.4. Construction of SSH cDNA Libraries
4.5. Agroinfiltration Experiments
4.6. GFP and mRFP Imaging
4.7. GFP Fluorescence Measurement
4.8. GUS Activity Measurement
4.9. Quantitative Real-Time PCR (qRT-PCR) Analysis of Transcript Concentrations
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sheshukova, E.V.; Ershova, N.M.; Lipskerov, F.A.; Komarova, T.V. Enhanced Synthesis of Foreign Nuclear Protein Stimulates Viral Reproduction via the Induction of γ-Thionin Expression. Plants 2022, 11, 1530. https://doi.org/10.3390/plants11121530
Sheshukova EV, Ershova NM, Lipskerov FA, Komarova TV. Enhanced Synthesis of Foreign Nuclear Protein Stimulates Viral Reproduction via the Induction of γ-Thionin Expression. Plants. 2022; 11(12):1530. https://doi.org/10.3390/plants11121530
Chicago/Turabian StyleSheshukova, Ekaterina V., Natalia M. Ershova, Fedor A. Lipskerov, and Tatiana V. Komarova. 2022. "Enhanced Synthesis of Foreign Nuclear Protein Stimulates Viral Reproduction via the Induction of γ-Thionin Expression" Plants 11, no. 12: 1530. https://doi.org/10.3390/plants11121530