Selected Papers from the 2nd International Electronic Conference on Plant Sciences

A special issue of Plants (ISSN 2223-7747).

Deadline for manuscript submissions: closed (15 June 2022) | Viewed by 4201

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Plant Physiology and Morphology Laboratory, Crop Science Department, Agricultural University of Athens, Lera Odos 75, 11855 Athens, Greece
Interests: crop nutrition dynamics; sulfur physiology; crop agronomic biofortification physiology through foliar application; phytoremediation physiology
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Division of Natural Drug Discovery, Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan
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Department of Biosciences, University of Milan, 20133 Milan, Italy
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Locality Feo di Vito, Department AGRARIA, University Mediterranea of Reggio Calabria, 89124 SNC Reggio Calabria, Italy
Interests: allelopathy; secondary metabolites; essential oils; weed management; plant nutrition; metabolomics; mode of action; chemical interaction; bio-herbicides
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Special Issue Information

Dear Colleagues,

This Special Issue of Plants (MDPI) collects Selected Papers from the 2nd International Electronic Conference on Plant Science. The SI is focused on deepening plant research at all levels, from the molecular through to the whole plant and community scale, also including bioinformatics and modeling approaches. Contributions will also cover new topics, such as plant–plant communication, protection of plant biodiversity, and plants in urban environments.

Equality and inclusion principles will be respected, as well as access to the wider and more diverse scientific community. We encourage submissions from scientists at all career stages and from all backgrounds and aim for an equal gender balance.

We look forward to receiving your contributions to this Special Issue and would like to thank you in advance for your active support.

Sincerely,

Prof. Dr. Adriano Sofo
Prof. Dr. Dimitris L. Bouranis
Dr. Suresh Awale
Dr. Giorgio Perrella
Dr. Fabrizio Araniti
Dr. Giedrė Samuolienė
Guest Editors

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Keywords

  • plant physiology, signaling, and communication
  • plant ecology, biodiversity, and developmental biology
  • plant response to stresses and changing environment
  • phytochemistry and phytoremediation, plants in urban ecosystems
  • plant nutrition and plant–soil–microorganism interactions

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Published Papers (1 paper)

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Research

11 pages, 1938 KiB  
Article
Enhanced Synthesis of Foreign Nuclear Protein Stimulates Viral Reproduction via the Induction of γ-Thionin Expression
by Ekaterina V. Sheshukova, Natalia M. Ershova, Fedor A. Lipskerov and Tatiana V. Komarova
Plants 2022, 11(12), 1530; https://doi.org/10.3390/plants11121530 - 7 Jun 2022
Cited by 4 | Viewed by 2609
Abstract
Plants are a promising platform for recombinant protein production. Here we propose a novel approach to increase the level of viral vector-mediated recombinant protein synthesis. This approach is based on the hypothesis that antiviral protection is weakened during the antibacterial cellular response. We [...] Read more.
Plants are a promising platform for recombinant protein production. Here we propose a novel approach to increase the level of viral vector-mediated recombinant protein synthesis. This approach is based on the hypothesis that antiviral protection is weakened during the antibacterial cellular response. We suggested that introduced to the cell foreign nuclear localized proteins, including effectors such as bacterial nucleomodulins, can interfere with the import of cellular nuclear proteins and launch antibacterial defense reactions, creating favorable conditions for cytoplasmic virus reproduction. Here, we performed synthesis of an artificial nuclear protein—red fluorescent protein (mRFP) fused with a nuclear localization sequence (NLS)—in plant cells as a mimetic of a bacterial effector. Superproduction of mRFP:NLS induced Nicotiana benthamiana γ-thionin (NbγThio) mRNA accumulation. Both NLS-containing protein synthesis and increased NbγThio expression stimulated reproduction of the viral vector based on the genome of crucifer-infecting tobacco mosaic virus (crTMV) in N. benthamiana leaves. We isolated the NbγThio gene promoter (PrγThio) and showed that PrγThio activity sharply increased in response to massive synthesis of GFP fused with NLS. We conclude that NLS-induced PrγThio activation and increased accumulation of Nbγthio mRNA led to the stimulation of GFP expression from crTMV: GFP vector in the transient expression system. Full article
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